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| | <StructureSection load='5bq9' size='340' side='right'caption='[[5bq9]], [[Resolution|resolution]] 2.28Å' scene=''> | | <StructureSection load='5bq9' size='340' side='right'caption='[[5bq9]], [[Resolution|resolution]] 2.28Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5bq9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Legph Legph]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BQ9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5BQ9 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5bq9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Legionella_pneumophila_subsp._pneumophila_str._Philadelphia_1 Legionella pneumophila subsp. pneumophila str. Philadelphia 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BQ9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5BQ9 FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lpg1496 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272624 LEGPH])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5bq9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bq9 OCA], [https://pdbe.org/5bq9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5bq9 RCSB], [https://www.ebi.ac.uk/pdbsum/5bq9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5bq9 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5bq9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bq9 OCA], [http://pdbe.org/5bq9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5bq9 RCSB], [http://www.ebi.ac.uk/pdbsum/5bq9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5bq9 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q5ZVE4_LEGPH Q5ZVE4_LEGPH] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Legph]] | + | [[Category: Legionella pneumophila subsp. pneumophila str. Philadelphia 1]] |
| - | [[Category: Chang, C]] | + | [[Category: Chang C]] |
| - | [[Category: Evdokimova, E]] | + | [[Category: Evdokimova E]] |
| - | [[Category: Joachimiak, A]] | + | [[Category: Joachimiak A]] |
| - | [[Category: Structural genomic]]
| + | [[Category: Morar M]] |
| - | [[Category: Morar, M]] | + | [[Category: Savchenko A]] |
| - | [[Category: Savchenko, A]] | + | |
| - | [[Category: Mcsg]]
| + | |
| - | [[Category: Psi-biology]]
| + | |
| - | [[Category: Unknown function]]
| + | |
| Structural highlights
Function
Q5ZVE4_LEGPH
Publication Abstract from PubMed
Legionella pneumophila, the intracellular pathogen that can cause severe pneumonia known as Legionnaire's disease, translocates close to 300 effectors inside the host cell using Dot/Icm type IVB secretion system. The structure and function for the majority of these effector proteins remains unknown. Here, we present the crystal structure of the L. pneumophila effector Lem10. The structure reveals a multidomain organization with the largest C-terminal domain showing strong structural similarity to the HD protein superfamily representatives. However, Lem10 lacks the catalytic His-Asp residue pair and does not show any in vitro phosphohydrolase enzymatic activity, typical for HD proteins. While the biological function of Lem10 remains elusive, our analysis shows that similar distinct features are shared by a significant number of HD domains found in Legionella proteins, including the SidE family of effectors known to play an important role during infection. Taken together our data point to the presence of a specific group of non-catalytic Legionella HD domains, dubbed LHDs, which are involved in pathogenesis. Proteins 2015;. (c) 2015 Wiley Periodicals, Inc.
Crystal structure of the Legionella pneumophila lem10 effector reveals a new member of the HD protein superfamily.,Morar M, Evdokimova E, Chang C, Ensminger AW, Savchenko A Proteins. 2015 Oct 1. doi: 10.1002/prot.24933. PMID:26426142[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Morar M, Evdokimova E, Chang C, Ensminger AW, Savchenko A. Crystal structure of the Legionella pneumophila lem10 effector reveals a new member of the HD protein superfamily. Proteins. 2015 Oct 1. doi: 10.1002/prot.24933. PMID:26426142 doi:http://dx.doi.org/10.1002/prot.24933
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