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| | <StructureSection load='5by2' size='340' side='right'caption='[[5by2]], [[Resolution|resolution]] 2.80Å' scene=''> | | <StructureSection load='5by2' size='340' side='right'caption='[[5by2]], [[Resolution|resolution]] 2.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5by2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Colp3 Colp3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BY2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5BY2 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5by2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Colwellia_psychrerythraea_34H Colwellia psychrerythraea 34H]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BY2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5BY2 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gmhA, CPS_4434 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=167879 COLP3])</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5by2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5by2 OCA], [https://pdbe.org/5by2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5by2 RCSB], [https://www.ebi.ac.uk/pdbsum/5by2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5by2 ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/D-sedoheptulose_7-phosphate_isomerase D-sedoheptulose 7-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.28 5.3.1.28] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5by2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5by2 OCA], [http://pdbe.org/5by2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5by2 RCSB], [http://www.ebi.ac.uk/pdbsum/5by2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5by2 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/GMHA_COLP3 GMHA_COLP3]] Catalyzes the isomerization of sedoheptulose 7-phosphate in D-glycero-D-manno-heptose 7-phosphate. | + | [https://www.uniprot.org/uniprot/GMHA_COLP3 GMHA_COLP3] Catalyzes the isomerization of sedoheptulose 7-phosphate in D-glycero-D-manno-heptose 7-phosphate. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Colp3]] | + | [[Category: Colwellia psychrerythraea 34H]] |
| - | [[Category: D-sedoheptulose 7-phosphate isomerase]]
| + | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Chang, J H]] | + | [[Category: Chang JH]] |
| - | [[Category: Do, H]] | + | [[Category: Do H]] |
| - | [[Category: Lee, J H]] | + | [[Category: Lee JH]] |
| - | [[Category: Yun, J S]] | + | [[Category: Yun JS]] |
| - | [[Category: Colwellia psychrerythraea strain 34h]]
| + | |
| - | [[Category: Cpsgmha]]
| + | |
| - | [[Category: Isomerase]]
| + | |
| - | [[Category: Psychrophile]]
| + | |
| - | [[Category: Sedoheptulose 7-phosphate isomerase]]
| + | |
| Structural highlights
Function
GMHA_COLP3 Catalyzes the isomerization of sedoheptulose 7-phosphate in D-glycero-D-manno-heptose 7-phosphate.
Publication Abstract from PubMed
The psychrophilic organism Colwellia psychrerythraea strain 34H produces extracellular polysaccharide substances to tolerate cold environments. Sedoheptulose 7-phosphate isomerase (GmhA) is essential for producing Dglycero-D-mannoheptose 7-phosphate, a key mediator in the lipopolysaccharide biosynthetic pathway. We determined the crystal structure of GmhA from C. psychrerythraea strain 34H (CpsGmhA, UniProtKB code: Q47VU0) at a resolution of 2.8 A. The tetrameric structure is similar to that of homologous GmhA structures. Interestingly, one of the catalytic residues, glutamate, which has been reported to be critical for the activity of other homologous GmhA enzymes, is replaced by a glutamine residue in the CpsGmhA protein. We also found differences in the conformations of several other catalytic residues. Extensive structural and sequence analyses reveal that CpsGmhA shows high similarity to Escherichia coli DnaA initiatorassociating protein A (DiaA). Therefore, the CpsGmhA structure reported here may provide insight into the structural and functional correlations between GmhA and DiaA among specific microorganisms.
Crystal Structure and Comparative Sequence Analysis of GmhA from Colwellia psychrerythraea Strain 34H Provides Insight into Functional Similarity with DiaA.,Do H, Yun JS, Lee CW, Choi YJ, Kim HY, Kim YJ, Park H, Chang JH, Lee JH Mol Cells. 2015 Nov 26. doi: 10.14348/molcells.2015.0191. PMID:26612680[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Do H, Yun JS, Lee CW, Choi YJ, Kim HY, Kim YJ, Park H, Chang JH, Lee JH. Crystal Structure and Comparative Sequence Analysis of GmhA from Colwellia psychrerythraea Strain 34H Provides Insight into Functional Similarity with DiaA. Mol Cells. 2015 Nov 26. doi: 10.14348/molcells.2015.0191. PMID:26612680 doi:http://dx.doi.org/10.14348/molcells.2015.0191
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