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5c21

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Revision as of 06:16, 7 June 2023

Crystal structure of native HlyD from E. coli

Structural highlights

5c21 is a 2 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

HLYDC_ECOLX Involved in the transport of hemolysin A.

Publication Abstract from PubMed

The protein toxin HlyA of Escherichia coli is exported without a periplasmic intermediate by the type I secretion system (T1SS). The T1SS is composed of an inner membrane ABC transporter HlyB, an outer-membrane channel protein TolC, and a membrane fusion protein HlyD. However, the assembly of the T1SS remains to be elucidated. In this study, we determine the crystal structure of a part of the C-terminal periplasmic domain of HlyD. The long alpha-helical domain consisting of three alpha helices and a lipoyl domain was identified in the crystal structure. Based on the HlyD structure, we modeled the hexameric assembly of HlyD with a long alpha-helical barrel, which formed a complex with TolC in an intermeshing cogwheel-to-cogwheel manner, as observed in tripartite RND-type drug efflux pumps. These observations provide a structural blueprint for understanding the type I secretion system in pathogenic Gram-negative bacteria.

Crystal Structure of a Soluble Fragment of the Membrane Fusion Protein HlyD in a Type I Secretion System of Gram-Negative Bacteria.,Kim JS, Song S, Lee M, Lee S, Lee K, Ha NC Structure. 2016 Jan 22. pii: S0969-2126(16)00005-8. doi:, 10.1016/j.str.2015.12.012. PMID:26833388^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kim JS, Song S, Lee M, Lee S, Lee K, Ha NC. Crystal Structure of a Soluble Fragment of the Membrane Fusion Protein HlyD in a Type I Secretion System of Gram-Negative Bacteria. Structure. 2016 Jan 22. pii: S0969-2126(16)00005-8. doi:, 10.1016/j.str.2015.12.012. PMID:26833388 doi:http://dx.doi.org/10.1016/j.str.2015.12.012

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5c21"

Categories: Escherichia coli | Large Structures | Ha NC | Kim JS | Yoon BY

@@ Line 3: / Line 3: @@
 <StructureSection load='5c21' size='340' side='right'caption='[[5c21]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5c21]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C21 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5C21 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5c21]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C21 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5C21 FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5c22|5c22]]</td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5c21 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c21 OCA], [https://pdbe.org/5c21 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5c21 RCSB], [https://www.ebi.ac.uk/pdbsum/5c21 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5c21 ProSAT]</span></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">hlyD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5c21 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c21 OCA], [http://pdbe.org/5c21 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5c21 RCSB], [http://www.ebi.ac.uk/pdbsum/5c21 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5c21 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/HLYDC_ECOLX HLYDC_ECOLX] Involved in the transport of hemolysin A.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 24: / Line 24: @@
 __TOC__
 </StructureSection>
-[[Category: Bacillus coli migula 1895]]
+[[Category: Escherichia coli]]
 [[Category: Large Structures]]
-[[Category: Ha, N C]]
+[[Category: Ha NC]]
-[[Category: Kim, J S]]
+[[Category: Kim JS]]
-[[Category: Yoon, B Y]]
+[[Category: Yoon BY]]
-[[Category: Abc transporter]]
-[[Category: Hlyd]]
-[[Category: Protein transport]]

5c21

From Proteopedia

Revision as of 06:16, 7 June 2023

Crystal structure of native HlyD from E. coli

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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