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| | <StructureSection load='5c8e' size='340' side='right'caption='[[5c8e]], [[Resolution|resolution]] 3.89Å' scene=''> | | <StructureSection load='5c8e' size='340' side='right'caption='[[5c8e]], [[Resolution|resolution]] 3.89Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5c8e]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Thet2 Thet2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C8E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5C8E FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5c8e]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB27 Thermus thermophilus HB27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C8E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5C8E FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=5AD:5-DEOXYADENOSINE'>5AD</scene>, <scene name='pdbligand=B12:COBALAMIN'>B12</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5AD:5-DEOXYADENOSINE'>5AD</scene>, <scene name='pdbligand=B12:COBALAMIN'>B12</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5c8a|5c8a]], [[5c8f|5c8f]], [[5c8d|5c8d]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5c8e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c8e OCA], [https://pdbe.org/5c8e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5c8e RCSB], [https://www.ebi.ac.uk/pdbsum/5c8e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5c8e ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TT_P0056 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=262724 THET2])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5c8e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c8e OCA], [http://pdbe.org/5c8e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5c8e RCSB], [http://www.ebi.ac.uk/pdbsum/5c8e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5c8e ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q746J7_THET2 Q746J7_THET2] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | | | |
| | ==See Also== | | ==See Also== |
| - | *[[Suggestions for new articles|Suggestions for new articles]] | + | *[[Transcriptional activator 3D structures|Transcriptional activator 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Thet2]] | + | [[Category: Thermus thermophilus HB27]] |
| - | [[Category: Drennan, C L]] | + | [[Category: Drennan CL]] |
| - | [[Category: Jost, M]] | + | [[Category: Jost M]] |
| - | [[Category: Adenosylcobalamin-binding]]
| + | |
| - | [[Category: Dna-binding]]
| + | |
| - | [[Category: Light sensor]]
| + | |
| - | [[Category: Transcription factor]]
| + | |
| - | [[Category: Transcription regulator-dna complex]]
| + | |
| Structural highlights
Function
Q746J7_THET2
Publication Abstract from PubMed
Photoreceptor proteins enable organisms to sense and respond to light. The newly discovered CarH-type photoreceptors use a vitamin B12 derivative, adenosylcobalamin, as the light-sensing chromophore to mediate light-dependent gene regulation. Here we present crystal structures of Thermus thermophilus CarH in all three relevant states: in the dark, both free and bound to operator DNA, and after light exposure. These structures provide visualizations of how adenosylcobalamin mediates CarH tetramer formation in the dark, how this tetramer binds to the promoter -35 element to repress transcription, and how light exposure leads to a large-scale conformational change that activates transcription. In addition to the remarkable functional repurposing of adenosylcobalamin from an enzyme cofactor to a light sensor, we find that nature also repurposed two independent protein modules in assembling CarH. These results expand the biological role of vitamin B12 and provide fundamental insight into a new mode of light-dependent gene regulation.
Structural basis for gene regulation by a B-dependent photoreceptor.,Jost M, Fernandez-Zapata J, Polanco MC, Ortiz-Guerrero JM, Chen PY, Kang G, Padmanabhan S, Elias-Arnanz M, Drennan CL Nature. 2015 Sep 28. doi: 10.1038/nature14950. PMID:26416754[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Jost M, Fernandez-Zapata J, Polanco MC, Ortiz-Guerrero JM, Chen PY, Kang G, Padmanabhan S, Elias-Arnanz M, Drennan CL. Structural basis for gene regulation by a B-dependent photoreceptor. Nature. 2015 Sep 28. doi: 10.1038/nature14950. PMID:26416754 doi:http://dx.doi.org/10.1038/nature14950
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