User:Francielle Aguiar Gomes/Sandbox 1
From Proteopedia
(Difference between revisions)
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==Photosynthetic LH1-RC Super-complex of ''Rhodospirillum rubrum''== | ==Photosynthetic LH1-RC Super-complex of ''Rhodospirillum rubrum''== | ||
| - | <StructureSection load='7EQD' size='340' side='right' caption=' | + | <StructureSection load='7EQD' size='340' side='right' caption='Photosynthetic LH1-RC Super-complex of ''Rhodospirillum rubrum''' scene=''> |
== Introduction == | == Introduction == | ||
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and pigment molecules,3−5 both complexes have been intensively studied as models of the bacterial antenna apparatus6 and as such have provided a wealth of information on mechanisms of light energy acquisition, pigment−protein interactions, and assembly of multicomponent complexes. <ref>10.1021/acs.biochem.1c00360</ref> | and pigment molecules,3−5 both complexes have been intensively studied as models of the bacterial antenna apparatus6 and as such have provided a wealth of information on mechanisms of light energy acquisition, pigment−protein interactions, and assembly of multicomponent complexes. <ref>10.1021/acs.biochem.1c00360</ref> | ||
| - | == | + | == Structural highlights == |
Structures of both purified LH1 and the RC-associated core complex (LH1-RC) of ''Rsp. rubrum'' have not been obtained at high resolution, and no RC atomic structure is known. | Structures of both purified LH1 and the RC-associated core complex (LH1-RC) of ''Rsp. rubrum'' have not been obtained at high resolution, and no RC atomic structure is known. | ||
[[Image:Structure.png|300px|left|thumb| Structure overview of the Rsp. rubrum LH1-RC complex. (a) Side view of the LH1-RC parallel to the membrane plane. (b) Top view of the LH1-RC from the periplasmic side of the membrane. (c) Tilted view of the cofactor arrangement. (d) Superposition of Cα carbons of the LH1 αβpolypeptides between Rsp. rubrum and Tch. tepidum (gray, PDB: 5Y5S). Color scheme: LH1-α, green; LH1-β, slate-blue; L-subunit, magenta; Msubunit, blue; BChl aG in LH1 and special pair, red sticks; Accessory BChl aG, cyan sticks; BPhe aG, light-pink sticks; Spirilloxanthin, yellow sticks; UQ10, blue sticks; RQ-10, green sticks; Fe, magenta ball. Phospholipids and detergents are omitted for clarity]] | [[Image:Structure.png|300px|left|thumb| Structure overview of the Rsp. rubrum LH1-RC complex. (a) Side view of the LH1-RC parallel to the membrane plane. (b) Top view of the LH1-RC from the periplasmic side of the membrane. (c) Tilted view of the cofactor arrangement. (d) Superposition of Cα carbons of the LH1 αβpolypeptides between Rsp. rubrum and Tch. tepidum (gray, PDB: 5Y5S). Color scheme: LH1-α, green; LH1-β, slate-blue; L-subunit, magenta; Msubunit, blue; BChl aG in LH1 and special pair, red sticks; Accessory BChl aG, cyan sticks; BPhe aG, light-pink sticks; Spirilloxanthin, yellow sticks; UQ10, blue sticks; RQ-10, green sticks; Fe, magenta ball. Phospholipids and detergents are omitted for clarity]] | ||
| - | == | + | == Cryo-EM == |
| + | Cryogenic electron microscopy (cryo-EM) is a cryomicroscopy technique applied to samples cooled to cryogenic temperatures. For biological samples, structure is preserved by embedding in a glassy ice environment. An aqueous sample is applied to a mesh grid and frozen by immersion in liquid ethane or a mixture of liquid ethane and propane <ref>10.1017/S1431927608080781</ref>. This technique has advanced dramatically to become a viable tool for high-resolution structural biology research. The ultimate outcome of a cryoEM study is an atomic model of a macromolecule or its complex with interacting partners. | ||
| - | == | + | == Structure of Photosynthetic LH1-RC Super-complex of ''Rhodospirillum rubrum'' == |
This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes. | This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes. | ||
| - | </StructureSection> | ||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 17:15, 8 June 2023
Photosynthetic LH1-RC Super-complex of Rhodospirillum rubrum
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