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==Function and Structure== | ==Function and Structure== | ||
| - | <StructureSection load=' | + | <StructureSection load='5DE5' size='340' side='right' caption='Caption for this structure' scene=''> |
| - | Fragile X messenger ribonucleoprotein (FMRP) is encoded by the fragile X messenger ribonucleoprotein 1 (FMR1) gene, located in the X chromossome, associated with the fragile X syndrome, Fragile X Tremor/Ataxia Syndrome (FXTAS) and Premature Ovarian Failure (POF1). The FMRP binds to RNAs and its own FMR1 transcripts, possibly a self-regulatory mechanism. A motif that is going to be explored is the RGG motif, that the protein uses to bind to guanine G-quadruplexes a structure that consists of nucleic acid folding in which four guanines arrange in a planar conformation stabilized by Hoogsteen-trype hydrogen bonds, named tetrad. | + | Fragile X messenger ribonucleoprotein (FMRP) is encoded by the fragile X messenger ribonucleoprotein 1 (FMR1) gene, located in the X chromossome, associated with the fragile X syndrome (FXS), Fragile X Tremor/Ataxia Syndrome (FXTAS) and Premature Ovarian Failure (POF1). The FMRP binds to RNAs and its own FMR1 transcripts, possibly a self-regulatory mechanism. A motif that is going to be explored is the RGG motif, that the protein uses to bind to guanine G-quadruplexes a structure that consists of nucleic acid folding in which four guanines arrange in a planar conformation stabilized by Hoogsteen-trype hydrogen bonds, named tetrad. The structure that is being represented on the right represents the FMRP RGG motif and the G-quadruplex secondary structure in the RNA. The protein structure was obtained by X-ray diffraction with a 3 Å resolution <ref>doi.org/10.1073/pnas.1515737112 |
'''RGG motif''' | '''RGG motif''' | ||
| - | + | Different domains and motifs mediate the RNA binding mechanism and the exon 15-encoded RGG (arginine - glycine - glycine) motif is one of them. The RGG motif is well conserved in vertebrates.To easily represent the RGG motif binding to a RNA, this motif will be highlitghted in the scene <scene name='96/969643/Rgg_motif_binding_to_rna/16'>Crystal structure of the complex between human FMRP RGG motif and G-quadruplex RNA.</scene>. Therefore, the understanding of the interaction RGG-RNA is important for the comprehension of the FMRP and FXS. | |
| - | Different domains and motifs mediate the RNA binding mechanism and the exon 15-encoded RGG (arginine - glycine - glycine) motif is one of them. | + | |
You may include any references to papers as in: the use of JSmol in Proteopedia <ref>DOI 10.1002/ijch.201300024</ref> or to the article describing Jmol <ref>PMID:21638687</ref> to the rescue. | You may include any references to papers as in: the use of JSmol in Proteopedia <ref>DOI 10.1002/ijch.201300024</ref> or to the article describing Jmol <ref>PMID:21638687</ref> to the rescue. | ||
Revision as of 18:26, 9 June 2023
Function and Structure
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References
- ↑ doi.org/10.1073/pnas.1515737112 RGG motif Different domains and motifs mediate the RNA binding mechanism and the exon 15-encoded RGG (arginine - glycine - glycine) motif is one of them. The RGG motif is well conserved in vertebrates.To easily represent the RGG motif binding to a RNA, this motif will be highlitghted in the scene . Therefore, the understanding of the interaction RGG-RNA is important for the comprehension of the FMRP and FXS. You may include any references to papers as in: the use of JSmol in Proteopedia <ref>DOI 10.1002/ijch.201300024</li> <li id="cite_note-1">[[#cite_ref-1|↑]] Herraez A. Biomolecules in the computer: Jmol to the rescue. Biochem Mol Biol Educ. 2006 Jul;34(4):255-61. doi: 10.1002/bmb.2006.494034042644. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/21638687 21638687] doi:[http://dx.doi.org/10.1002/bmb.2006.494034042644 10.1002/bmb.2006.494034042644]</li></ol></ref>
