1l2t
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1l2t.gif|left|200px]] | [[Image:1l2t.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1l2t", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | | | + | --> |
| - | + | {{STRUCTURE_1l2t| PDB=1l2t | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''Dimeric Structure of MJ0796, a Bacterial ABC Transporter Cassette''' | '''Dimeric Structure of MJ0796, a Bacterial ABC Transporter Cassette''' | ||
| Line 29: | Line 26: | ||
[[Category: Rosen, J.]] | [[Category: Rosen, J.]] | ||
[[Category: Smith, P C.]] | [[Category: Smith, P C.]] | ||
| - | [[Category: | + | [[Category: Abc transporter]] |
| - | [[Category: | + | [[Category: Atpase]] |
| - | [[Category: | + | [[Category: Nbd]] |
| - | [[Category: | + | [[Category: Walker-a]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:28:40 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 20:28, 2 May 2008
Dimeric Structure of MJ0796, a Bacterial ABC Transporter Cassette
Overview
It has been proposed that the reaction cycle of ATP binding cassette (ABC) transporters is driven by dimerization of their ABC motor domains upon binding ATP at their mutual interface. However, no such ATP sandwich complex has been observed for an ABC from an ABC transporter. In this paper, we report the crystal structure of a stable dimer formed by the E171Q mutant of the MJ0796 ABC, which is hydrolytically inactive due to mutation of the catalytic base. The structure shows a symmetrical dimer in which two ATP molecules are each sandwiched between the Walker A motif in one subunit and the LSGGQ signature motif in the other subunit. These results establish the stereochemical basis of the power stroke of ABC transporter pumps.
About this Structure
1L2T is a Single protein structure of sequence from Methanocaldococcus jannaschii. Full crystallographic information is available from OCA.
Reference
ATP binding to the motor domain from an ABC transporter drives formation of a nucleotide sandwich dimer., Smith PC, Karpowich N, Millen L, Moody JE, Rosen J, Thomas PJ, Hunt JF, Mol Cell. 2002 Jul;10(1):139-49. PMID:12150914 Page seeded by OCA on Fri May 2 23:28:40 2008
