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| | ==Solution Structure of Myxoma Virus Protein M156R== | | ==Solution Structure of Myxoma Virus Protein M156R== |
| - | <StructureSection load='1jjg' size='340' side='right'caption='[[1jjg]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='1jjg' size='340' side='right'caption='[[1jjg]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1jjg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Myxv Myxv]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JJG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JJG FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1jjg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Myxoma_virus_(strain_Lausanne) Myxoma virus (strain Lausanne)]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JJG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JJG FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">M156R ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=31530 MYXV])</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jjg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jjg OCA], [https://pdbe.org/1jjg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jjg RCSB], [https://www.ebi.ac.uk/pdbsum/1jjg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jjg ProSAT], [https://www.topsan.org/Proteins/NESGC/1jjg TOPSAN]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jjg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jjg OCA], [https://pdbe.org/1jjg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jjg RCSB], [https://www.ebi.ac.uk/pdbsum/1jjg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jjg ProSAT], [https://www.topsan.org/Proteins/NESGC/1jjg TOPSAN]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q9Q8E9_MYXVL Q9Q8E9_MYXVL] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Myxv]]
| + | [[Category: Arrowsmith CH]] |
| - | [[Category: Arrowsmith, C H]] | + | [[Category: Cort JR]] |
| - | [[Category: Cort, J R]] | + | [[Category: Kennedy MA]] |
| - | [[Category: Kennedy, M A]] | + | [[Category: Ramelot TA]] |
| - | [[Category: Structural genomic]]
| + | [[Category: Yee AA]] |
| - | [[Category: Ramelot, T A]] | + | |
| - | [[Category: Yee, A A]] | + | |
| - | [[Category: Beta barrel]]
| + | |
| - | [[Category: Eif-2a homolog]]
| + | |
| - | [[Category: Myxv156r]]
| + | |
| - | [[Category: Nesg project]]
| + | |
| - | [[Category: Ob fold]]
| + | |
| - | [[Category: PSI, Protein structure initiative]]
| + | |
| - | [[Category: S1 motif]]
| + | |
| - | [[Category: Viral protein]]
| + | |
| Structural highlights
Function
Q9Q8E9_MYXVL
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Phosphorylation of the translation initiation factor eIF2 on Ser51 of its alpha subunit is a key event for regulation of protein synthesis in all eukaryotes. M156R, the product of the myxoma virus M156R open reading frame, has sequence similarity to eIF2alpha as well as to a family of viral proteins that bind to the interferon-induced protein kinase PKR and inhibit phosphorylation of eIF2alpha. In this study, we demonstrate that, like eIF2alpha. M156R is an efficient substrate for phosphorylation by PKR and can compete with eIF2alpha. To gain insights into the substrate specificity of the eIF2alpha kinases, we have determined the nuclear magnetic resonance (NMR) structure of M156R, the first structure of a myxoma virus protein. The fold consists of a five-stranded antiparallel beta-barrel with two of the strands connected by a loop and an alpha-helix. The similarity between M156R and the beta-barrel structure in the N terminus of eIF2alpha suggests that the viral homologs mimic eIF2alpha structure in order to compete for binding to PKR. A homology-modeled structure of the well-studied vaccinia virus K3L was generated on the basis of alignment with M156R. Comparison of the structures of the K3L model, M156R, and human eIF2alpha indicated that residues important for binding to PKR are located at conserved positions on the surface of the beta-barrel and in the mobile loop, identifying the putative PKR recognition motif.
Myxoma virus immunomodulatory protein M156R is a structural mimic of eukaryotic translation initiation factor eIF2alpha.,Ramelot TA, Cort JR, Yee AA, Liu F, Goshe MB, Edwards AM, Smith RD, Arrowsmith CH, Dever TE, Kennedy MA J Mol Biol. 2002 Oct 4;322(5):943-54. PMID:12367520[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Ramelot TA, Cort JR, Yee AA, Liu F, Goshe MB, Edwards AM, Smith RD, Arrowsmith CH, Dever TE, Kennedy MA. Myxoma virus immunomodulatory protein M156R is a structural mimic of eukaryotic translation initiation factor eIF2alpha. J Mol Biol. 2002 Oct 4;322(5):943-54. PMID:12367520
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