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| | ==Antibacterial Peptide from Eggshell Matrix: Structure and Self-assembly of beta-defensin Like Peptide from the Chinese Soft-shelled Turtle Eggshell== | | ==Antibacterial Peptide from Eggshell Matrix: Structure and Self-assembly of beta-defensin Like Peptide from the Chinese Soft-shelled Turtle Eggshell== |
| - | <StructureSection load='2jr3' size='340' side='right'caption='[[2jr3]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2jr3' size='340' side='right'caption='[[2jr3]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| | <table><tr><td colspan='2'>[[2jr3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pelodiscus_sinensis Pelodiscus sinensis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JR3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JR3 FirstGlance]. <br> | | <table><tr><td colspan='2'>[[2jr3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pelodiscus_sinensis Pelodiscus sinensis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JR3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JR3 FirstGlance]. <br> |
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| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/PVAT_PELSI PVAT_PELSI]] Induces the nucleation and stabilization of vaterite, one of the crystalline polymorphs of calcium carbonate. Exhibits strong antimicrobial activity against Pseudomonas aeruginosa and Proteus vulgaris.<ref>PMID:15877362</ref> <ref>PMID:18341335</ref>
| + | [https://www.uniprot.org/uniprot/PVAT_PELSI PVAT_PELSI] Induces the nucleation and stabilization of vaterite, one of the crystalline polymorphs of calcium carbonate. Exhibits strong antimicrobial activity against Pseudomonas aeruginosa and Proteus vulgaris.<ref>PMID:15877362</ref> <ref>PMID:18341335</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| | [[Category: Pelodiscus sinensis]] | | [[Category: Pelodiscus sinensis]] |
| - | [[Category: Banerjee, Y]] | + | [[Category: Banerjee Y]] |
| - | [[Category: Chi-Jin, E O]] | + | [[Category: Chi-Jin EO]] |
| - | [[Category: Jois, S D.S]] | + | [[Category: Jois SDS]] |
| - | [[Category: Kini, R M]] | + | [[Category: Kini RM]] |
| - | [[Category: Lakshminarayanan, R]] | + | [[Category: Lakshminarayanan R]] |
| - | [[Category: Samy, R Perumal]] | + | [[Category: Perumal Samy R]] |
| - | [[Category: Teo, K W]] | + | [[Category: Teo KW]] |
| - | [[Category: Valiyaveettil, S]] | + | [[Category: Valiyaveettil S]] |
| - | [[Category: Vivekanandan, S]] | + | [[Category: Vivekanandan S]] |
| - | [[Category: Antibacterial peptide]]
| + | |
| - | [[Category: Antimicrobial protein]]
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| - | [[Category: Beta-defensin like peptide]]
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| - | [[Category: Chinese soft-shelled turtle eggshell]]
| + | |
| - | [[Category: Eggshell matrix]]
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| Structural highlights
Function
PVAT_PELSI Induces the nucleation and stabilization of vaterite, one of the crystalline polymorphs of calcium carbonate. Exhibits strong antimicrobial activity against Pseudomonas aeruginosa and Proteus vulgaris.[1] [2]
Publication Abstract from PubMed
Biomineral matrix formation and molecular recognition are two important processes associated with eggshell biomineralization. To understand these two processes, a major intracrystalline peptide, pelovaterin, was isolated from turtle (Pelodiscus sinensis) eggshell and its tertiary and quaternary structures were established. The global fold of pelovaterin is similar to that of human beta-defensins but has a large hydrophobic core and a short hydrophilic N-terminal segment, which is not preserved in defensins. Pelovaterin exhibits strong antimicrobial activity against two pathogenic gram-negative bacteria, Pseudomonas aeruginosa and Proteus vulgaris, and stabilizes a thin film of metastable vaterite. We show that pelovaterin self-aggregates in the form of micellar nanospheres and the aggregation in solution is entropy-driven. It is suggested that the micellar aggregation of pelovaterin is responsible for the induction and stabilization of the metastable phase by altering the interfacial energy. The results demonstrate the adaptability of an extracellular matrix protein to perform multiple tasks: polymorph discrimination and protection of the contents of the egg against bacterial invasion.
Structure, self-assembly, and dual role of a beta-defensin-like peptide from the Chinese soft-shelled turtle eggshell matrix.,Lakshminarayanan R, Vivekanandan S, Samy RP, Banerjee Y, Chi-Jin EO, Teo KW, Jois SD, Kini RM, Valiyaveettil S J Am Chem Soc. 2008 Apr 9;130(14):4660-8. Epub 2008 Mar 15. PMID:18341335[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Lakshminarayanan R, Chi-Jin EO, Loh XJ, Kini RM, Valiyaveettil S. Purification and characterization of a vaterite-inducing peptide, pelovaterin, from the eggshells of Pelodiscus sinensis (Chinese soft-shelled turtle). Biomacromolecules. 2005 May-Jun;6(3):1429-37. PMID:15877362 doi:http://dx.doi.org/10.1021/bm049276f
- ↑ Lakshminarayanan R, Vivekanandan S, Samy RP, Banerjee Y, Chi-Jin EO, Teo KW, Jois SD, Kini RM, Valiyaveettil S. Structure, self-assembly, and dual role of a beta-defensin-like peptide from the Chinese soft-shelled turtle eggshell matrix. J Am Chem Soc. 2008 Apr 9;130(14):4660-8. Epub 2008 Mar 15. PMID:18341335 doi:10.1021/ja075659k
- ↑ Lakshminarayanan R, Vivekanandan S, Samy RP, Banerjee Y, Chi-Jin EO, Teo KW, Jois SD, Kini RM, Valiyaveettil S. Structure, self-assembly, and dual role of a beta-defensin-like peptide from the Chinese soft-shelled turtle eggshell matrix. J Am Chem Soc. 2008 Apr 9;130(14):4660-8. Epub 2008 Mar 15. PMID:18341335 doi:10.1021/ja075659k
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