1l3d
From Proteopedia
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'''Low Resolution Crystal Structure of a Viral RNA Pseudoknot''' | '''Low Resolution Crystal Structure of a Viral RNA Pseudoknot''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L3D OCA]. | |
==Reference== | ==Reference== | ||
Metal ions and flexibility in a viral RNA pseudoknot at atomic resolution., Egli M, Minasov G, Su L, Rich A, Proc Natl Acad Sci U S A. 2002 Apr 2;99(7):4302-7. Epub 2002 Mar 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11904368 11904368] | Metal ions and flexibility in a viral RNA pseudoknot at atomic resolution., Egli M, Minasov G, Su L, Rich A, Proc Natl Acad Sci U S A. 2002 Apr 2;99(7):4302-7. Epub 2002 Mar 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11904368 11904368] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Egli, M.]] | [[Category: Egli, M.]] | ||
[[Category: Minasov, G.]] | [[Category: Minasov, G.]] | ||
[[Category: Rich, A.]] | [[Category: Rich, A.]] | ||
[[Category: Su, L.]] | [[Category: Su, L.]] | ||
| - | [[Category: | + | [[Category: Flexibility]] |
| - | [[Category: | + | [[Category: Frameshifting]] |
| - | [[Category: | + | [[Category: Pseudoknot]] |
| - | [[Category: | + | [[Category: Viral rna]] |
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 20:29, 2 May 2008
Low Resolution Crystal Structure of a Viral RNA Pseudoknot
Overview
Many pathogenic viruses use programmed -1 ribosomal frameshifting to regulate translation of their structural and enzymatic proteins from polycistronic mRNAs. Frameshifting is commonly stimulated by a pseudoknot located downstream from a slippery sequence, the latter positioned at the ribosomal A and P sites. We report here the structures of two crystal forms of the frameshifting RNA pseudoknot from beet western yellow virus at resolutions of 1.25 and 2.85 A. Because of the very high resolution of 1.25 A, ten mono- and divalent metal ions per asymmetric unit could be identified, giving insight into potential roles of metal ions in stabilizing the pseudoknot. A magnesium ion located at the junction of the two pseudoknot stems appears to play a crucial role in stabilizing the structure. Because the two crystal forms exhibit mostly unrelated packing interactions and local crystallographic disorder in the high-resolution form was resolvable, the two structures offer the most detailed view yet of the conformational preference and flexibility of an RNA pseudoknot.
About this Structure
Full crystallographic information is available from OCA.
Reference
Metal ions and flexibility in a viral RNA pseudoknot at atomic resolution., Egli M, Minasov G, Su L, Rich A, Proc Natl Acad Sci U S A. 2002 Apr 2;99(7):4302-7. Epub 2002 Mar 19. PMID:11904368 Page seeded by OCA on Fri May 2 23:29:36 2008
Categories: Egli, M. | Minasov, G. | Rich, A. | Su, L. | Flexibility | Frameshifting | Pseudoknot | Viral rna
