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| | ==NMR solution structure of TM0212 at 40 C== | | ==NMR solution structure of TM0212 at 40 C== |
| - | <StructureSection load='2ka7' size='340' side='right'caption='[[2ka7]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2ka7' size='340' side='right'caption='[[2ka7]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2ka7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_43589 Atcc 43589]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KA7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KA7 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2ka7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KA7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KA7 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1zko|1zko]]</div></td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ka7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ka7 OCA], [https://pdbe.org/2ka7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ka7 RCSB], [https://www.ebi.ac.uk/pdbsum/2ka7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ka7 ProSAT], [https://www.topsan.org/Proteins/JCSG/2ka7 TOPSAN]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gcvH, TM_0212 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2336 ATCC 43589])</td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ka7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ka7 OCA], [https://pdbe.org/2ka7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ka7 RCSB], [https://www.ebi.ac.uk/pdbsum/2ka7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ka7 ProSAT], [https://www.topsan.org/Proteins/JCSG/2ka7 TOPSAN]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/GCSH_THEMA GCSH_THEMA]] The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein (By similarity).
| + | [https://www.uniprot.org/uniprot/GCSH_THEMA GCSH_THEMA] The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein (By similarity). |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 43589]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Geralt, M]] | + | [[Category: Thermotoga maritima]] |
| - | [[Category: Herrmann, T]] | + | [[Category: Geralt M]] |
| - | [[Category: Structural genomic]] | + | [[Category: Herrmann T]] |
| - | [[Category: Mohanty, B]] | + | [[Category: Mohanty B]] |
| - | [[Category: Pedrini, B]] | + | [[Category: Pedrini B]] |
| - | [[Category: Wilson, I]] | + | [[Category: Wilson I]] |
| - | [[Category: Wuthrich, K]] | + | [[Category: Wuthrich K]] |
| - | [[Category: Jcsg]]
| + | |
| - | [[Category: Lipoyl]]
| + | |
| - | [[Category: Protein]]
| + | |
| - | [[Category: PSI, Protein structure initiative]]
| + | |
| - | [[Category: Unknown function]]
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| Structural highlights
Function
GCSH_THEMA The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein (By similarity).
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The J-UNIO (JCSG protocol using the software UNIO) procedure for automated protein structure determination by NMR in solution is introduced. In the present implementation, J-UNIO makes use of APSY-NMR spectroscopy, 3D heteronuclear-resolved [(1)H,(1)H]-NOESY experiments, and the software UNIO. Applications with proteins from the JCSG target list with sizes up to 150 residues showed that the procedure is highly robust and efficient. In all instances the correct polypeptide fold was obtained in the first round of automated data analysis and structure calculation. After interactive validation of the data obtained from the automated routine, the quality of the final structures was comparable to results from interactive structure determination. Special advantages are that the NMR data have been recorded with 6-10 days of instrument time per protein, that there is only a single step of chemical shift adjustments to relate the backbone signals in the APSY-NMR spectra with the corresponding backbone signals in the NOESY spectra, and that the NOE-based amino acid side chain chemical shift assignments are automatically focused on those residues that are heavily weighted in the structure calculation. The individual working steps of J-UNIO are illustrated with the structure determination of the protein YP_926445.1 from Shewanella amazonensis, and the results obtained with 17 JCSG targets are critically evaluated.
The J-UNIO protocol for automated protein structure determination by NMR in solution.,Serrano P, Pedrini B, Mohanty B, Geralt M, Herrmann T, Wuthrich K J Biomol NMR. 2012 Aug;53(4):341-54. doi: 10.1007/s10858-012-9645-2. Epub 2012, Jul 3. PMID:22752932[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Serrano P, Pedrini B, Mohanty B, Geralt M, Herrmann T, Wuthrich K. The J-UNIO protocol for automated protein structure determination by NMR in solution. J Biomol NMR. 2012 Aug;53(4):341-54. doi: 10.1007/s10858-012-9645-2. Epub 2012, Jul 3. PMID:22752932 doi:http://dx.doi.org/10.1007/s10858-012-9645-2
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