1l5a

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[[Image:1l5a.gif|left|200px]]
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{{Structure
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|PDB= 1l5a |SIZE=350|CAPTION= <scene name='initialview01'>1l5a</scene>, resolution 2.55&Aring;
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The line below this paragraph, containing "STRUCTURE_1l5a", creates the "Structure Box" on the page.
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|GENE= vibH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 Vibrio cholerae])
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{{STRUCTURE_1l5a| PDB=1l5a | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1l5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l5a OCA], [http://www.ebi.ac.uk/pdbsum/1l5a PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1l5a RCSB]</span>
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'''Crystal Structure of VibH, an NRPS Condensation Enzyme'''
'''Crystal Structure of VibH, an NRPS Condensation Enzyme'''
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[[Category: Marshall, C G.]]
[[Category: Marshall, C G.]]
[[Category: Walsh, C T.]]
[[Category: Walsh, C T.]]
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[[Category: amide synthase]]
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[[Category: Amide synthase]]
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[[Category: nonribosomal peptide synthetase]]
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[[Category: Nonribosomal peptide synthetase]]
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[[Category: nrps condensation domain]]
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[[Category: Nrps condensation domain]]
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[[Category: vibriobactin]]
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[[Category: Vibriobactin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:33:12 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:58:13 2008''
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Revision as of 20:33, 2 May 2008

Template:STRUCTURE 1l5a

Crystal Structure of VibH, an NRPS Condensation Enzyme


Overview

Nonribosomal peptide synthetases (NRPSs) are large, multidomain enzymes that biosynthesize medically important natural products. We report the crystal structure of the free-standing NRPS condensation (C) domain VibH, which catalyzes amide bond formation in the synthesis of vibriobactin, a Vibrio cholerae siderophore. Despite low sequence identity, NRPS condensation enzymes are structurally related to chloramphenicol acetyltransferase (CAT) and dihydrolipoamide acyltransferases. However, although the latter enzymes are homotrimers, VibH is a monomeric pseudodimer. The VibH structure is representative of both NRPS condensation and epimerization domains, as well as the condensation-variant cyclization domains, which are all expected to be monomers. Surprisingly, despite favorable positioning in the active site, a universally conserved histidine important in CAT and in other C domains is not critical for general base catalysis in VibH.

About this Structure

1L5A is a Single protein structure of sequence from Vibrio cholerae. Full crystallographic information is available from OCA.

Reference

The structure of VibH represents nonribosomal peptide synthetase condensation, cyclization and epimerization domains., Keating TA, Marshall CG, Walsh CT, Keating AE, Nat Struct Biol. 2002 Jul;9(7):522-6. PMID:12055621 Page seeded by OCA on Fri May 2 23:33:12 2008

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