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| | ==Solution structure of jerdostatin mutant R24K from Trimeresurus jerdonii== | | ==Solution structure of jerdostatin mutant R24K from Trimeresurus jerdonii== |
| - | <StructureSection load='2w9u' size='340' side='right'caption='[[2w9u]], [[NMR_Ensembles_of_Models | 42 NMR models]]' scene=''> | + | <StructureSection load='2w9u' size='340' side='right'caption='[[2w9u]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2w9u]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W9U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W9U FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2w9u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Protobothrops_jerdonii Protobothrops jerdonii]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W9U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W9U FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2w9o|2w9o]], [[2w9v|2w9v]], [[2w9w|2w9w]]</div></td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w9u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w9u OCA], [https://pdbe.org/2w9u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w9u RCSB], [https://www.ebi.ac.uk/pdbsum/2w9u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w9u ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w9u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w9u OCA], [https://pdbe.org/2w9u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w9u RCSB], [https://www.ebi.ac.uk/pdbsum/2w9u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w9u ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/DIS_PROJR DIS_PROJR] Recombinant protein inhibits the adhesion of alpha-1/beta-1-K562 (ITGA1/ITGB1) cells to collagen IV with an IC(50) of 80 nM.<ref>PMID:16215260</ref> <ref>PMID:21656569</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Calvete, J J]] | + | [[Category: Protobothrops jerdonii]] |
| - | [[Category: Carbajo, R J]] | + | [[Category: Calvete JJ]] |
| - | [[Category: Mosulen, S]] | + | [[Category: Carbajo RJ]] |
| - | [[Category: Pineda-Lucena, A]] | + | [[Category: Mosulen S]] |
| - | [[Category: Sanz, L]] | + | [[Category: Pineda-Lucena A]] |
| - | [[Category: Blood coagulation]]
| + | [[Category: Sanz L]] |
| - | [[Category: Cell adhesion]]
| + | |
| - | [[Category: Toxin]]
| + | |
| - | [[Category: Venom]]
| + | |
| Structural highlights
Function
DIS_PROJR Recombinant protein inhibits the adhesion of alpha-1/beta-1-K562 (ITGA1/ITGB1) cells to collagen IV with an IC(50) of 80 nM.[1] [2]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
NMR analysis of four recombinant jerdostatin molecules was assessed to define the structural basis of two naturally occurring gain-of-function events: C-terminal dipeptide processing and mutation of the active residue K21 to arginine. Removal of the highly mobile and a bulky C-terminal dipeptide produced pronounced chemical shift changes in the sequentially unconnected but spatially nearby alpha(1)beta(1) inhibitory loop. Analysis of chemical shift divergence and (15)N backbone relaxation dynamics indicated differences in motions in the picosecond to nanosecond time scale, and the higher T(2) rate of S25, S26, and H27 of rJerK21 point to a slowdown in the microsecond to millisecond motions of these residues when compared with rJerR21. The evidence presented in this article converges on the hypothesis that dynamic differences between the alpha(1)beta(1) recognition loops of rJerR21 and rJerK21 may influence the thermodynamics of their receptor recognition and binding. A decrease in the mus-ms time scale may impair the binding affinity by reducing the rate of possible conformations that the rJerK21 can adopt in this time scale.
NMR structure and dynamics of recombinant wild type and mutated jerdostatin, a selective inhibitor of integrin alpha1beta1.,Carbajo RJ, Sanz L, Mosulen S, Perez A, Marcinkiewicz C, Pineda-Lucena A, Calvete JJ Proteins. 2011 Aug;79(8):2530-42. doi: 10.1002/prot.23076. Epub 2011 Jun, 7. PMID:21656569[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Sanz L, Chen RQ, Pérez A, Hilario R, Juárez P, Marcinkiewicz C, Monleón D, Celda B, Xiong YL, Pérez-Payá E, Calvete JJ. cDNA cloning and functional expression of jerdostatin, a novel RTS-disintegrin from Trimeresurus jerdonii and a specific antagonist of the alpha1beta1 integrin. J Biol Chem. 2005 Dec 9;280(49):40714-22. PMID:16215260 doi:10.1074/jbc.M509738200
- ↑ Carbajo RJ, Sanz L, Mosulen S, Perez A, Marcinkiewicz C, Pineda-Lucena A, Calvete JJ. NMR structure and dynamics of recombinant wild type and mutated jerdostatin, a selective inhibitor of integrin alpha1beta1. Proteins. 2011 Aug;79(8):2530-42. doi: 10.1002/prot.23076. Epub 2011 Jun, 7. PMID:21656569 doi:10.1002/prot.23076
- ↑ Carbajo RJ, Sanz L, Mosulen S, Perez A, Marcinkiewicz C, Pineda-Lucena A, Calvete JJ. NMR structure and dynamics of recombinant wild type and mutated jerdostatin, a selective inhibitor of integrin alpha1beta1. Proteins. 2011 Aug;79(8):2530-42. doi: 10.1002/prot.23076. Epub 2011 Jun, 7. PMID:21656569 doi:10.1002/prot.23076
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