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| | ==NMR solution structure of factor I-like modules of complement C7.== | | ==NMR solution structure of factor I-like modules of complement C7.== |
| - | <StructureSection load='2wcy' size='340' side='right'caption='[[2wcy]], [[NMR_Ensembles_of_Models | 48 NMR models]]' scene=''> | + | <StructureSection load='2wcy' size='340' side='right'caption='[[2wcy]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2wcy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WCY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WCY FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2wcy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WCY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WCY FirstGlance]. <br> |
| | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wcy OCA], [https://pdbe.org/2wcy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wcy RCSB], [https://www.ebi.ac.uk/pdbsum/2wcy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wcy ProSAT]</span></td></tr> | | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wcy OCA], [https://pdbe.org/2wcy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wcy RCSB], [https://www.ebi.ac.uk/pdbsum/2wcy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wcy ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Disease == |
| | + | [https://www.uniprot.org/uniprot/CO7_HUMAN CO7_HUMAN] Immunodeficiency due to a late component of complement deficiency. Disease susceptibility is associated with variations affecting the gene represented in this entry. |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/CO7_HUMAN CO7_HUMAN] Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C7 serves as a membrane anchor. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Barlow, P N]] | + | [[Category: Barlow PN]] |
| - | [[Category: Bramham, J]] | + | [[Category: Bramham J]] |
| - | [[Category: Ogata, R T]] | + | [[Category: Ogata RT]] |
| - | [[Category: Phelan, M M]] | + | [[Category: Phelan MM]] |
| - | [[Category: Soares, D C]] | + | [[Category: Soares DC]] |
| - | [[Category: Thai, C T]] | + | [[Category: Thai CT]] |
| - | [[Category: C7]]
| + | |
| - | [[Category: Complement]]
| + | |
| - | [[Category: Complement alternate pathway]]
| + | |
| - | [[Category: Complement pathway]]
| + | |
| - | [[Category: Cytolysis]]
| + | |
| - | [[Category: Disease mutation]]
| + | |
| - | [[Category: Disulfide]]
| + | |
| - | [[Category: Disulfide bond]]
| + | |
| - | [[Category: Egf]]
| + | |
| - | [[Category: Egf-like domain]]
| + | |
| - | [[Category: Factor i module]]
| + | |
| - | [[Category: Fim]]
| + | |
| - | [[Category: Fimac]]
| + | |
| - | [[Category: Follistatin]]
| + | |
| - | [[Category: Foln]]
| + | |
| - | [[Category: Glycoprotein]]
| + | |
| - | [[Category: Immune response]]
| + | |
| - | [[Category: Immune system]]
| + | |
| - | [[Category: Innate immunity]]
| + | |
| - | [[Category: Kazal]]
| + | |
| - | [[Category: Mac]]
| + | |
| - | [[Category: Membrane attack complex]]
| + | |
| - | [[Category: Polymorphism]]
| + | |
| - | [[Category: Secreted]]
| + | |
| - | [[Category: Sushi]]
| + | |
| Structural highlights
Disease
CO7_HUMAN Immunodeficiency due to a late component of complement deficiency. Disease susceptibility is associated with variations affecting the gene represented in this entry.
Function
CO7_HUMAN Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C7 serves as a membrane anchor.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Factor I-like modules (FIMs) of complement proteins C6, C7, and factor I participate in protein-protein interactions critical to the progress of a complement-mediated immune response to infections and other trauma. For instance, the carboxyl-terminal FIM pair of C7 (C7-FIMs) binds to the C345C domain of C5 and its activated product, C5b, during self-assembly of the cytolytic membrane-attack complex. FIMs share sequence similarity with follistatin domains (FDs) of known three-dimensional structure, suggesting that FIM structures could be reliably modeled. However, conflicting disulfide maps, inconsistent orientations of subdomains within FDs, and the presence of binding partners in all FD structures led us to determine the three-dimensional structure of C7-FIMs by NMR spectroscopy. The solution structure reveals that each FIM within C7 contains a small amino-terminal FOLN subdomain connected to a larger carboxyl-terminal KAZAL domain. The open arrangement of the subdomains within FIMs resembles that of first FDs within structures of tandem FDs but differs from the more compact subdomain arrangement of second or third FDs. Unexpectedly, the two C7-FIMs pack closely together with an approximate 2-fold rotational symmetry that is rarely seen in module pairs and has not been observed in FD-containing proteins. Interfaces between subdomains and between modules include numerous hydrophobic and electrostatic contributions, suggesting that this is a physiologically relevant conformation that persists in the context of the parent protein. Similar interfaces were predicted in a homology-based model of the C6-FIM pair. The C7-FIM structures also facilitated construction of a model of the single FIM of factor I.
Solution structure of factor I-like modules from complement C7 reveals a pair of follistatin domains in compact pseudosymmetric arrangement.,Phelan MM, Thai CT, Soares DC, Ogata RT, Barlow PN, Bramham J J Biol Chem. 2009 Jul 17;284(29):19637-49. Epub 2009 May 6. PMID:19419965[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Phelan MM, Thai CT, Soares DC, Ogata RT, Barlow PN, Bramham J. Solution structure of factor I-like modules from complement C7 reveals a pair of follistatin domains in compact pseudosymmetric arrangement. J Biol Chem. 2009 Jul 17;284(29):19637-49. Epub 2009 May 6. PMID:19419965 doi:10.1074/jbc.M901993200
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