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| | ==The C-terminal domain of rice beta-galactosidase 1== | | ==The C-terminal domain of rice beta-galactosidase 1== |
| - | <StructureSection load='5iaz' size='340' side='right'caption='[[5iaz]], [[NMR_Ensembles_of_Models | 25 NMR models]]' scene=''> | + | <StructureSection load='5iaz' size='340' side='right'caption='[[5iaz]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5iaz]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Indian_rice Indian rice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IAZ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5IAZ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5iaz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryza_sativa_Indica_Group Oryza sativa Indica Group]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IAZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5IAZ FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">OsI_10152 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=39946 Indian rice])</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5iaz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5iaz OCA], [https://pdbe.org/5iaz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5iaz RCSB], [https://www.ebi.ac.uk/pdbsum/5iaz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5iaz ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5iaz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5iaz OCA], [http://pdbe.org/5iaz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5iaz RCSB], [http://www.ebi.ac.uk/pdbsum/5iaz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5iaz ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/B8ANX7_ORYSI B8ANX7_ORYSI] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Indian rice]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Hua, Y l]] | + | [[Category: Oryza sativa Indica Group]] |
| - | [[Category: Ketudat-Cairns, J R]] | + | [[Category: Hua Y-l]] |
| - | [[Category: Rimlumduan, T]] | + | [[Category: Ketudat-Cairns JR]] |
| - | [[Category: Tanaka, T]] | + | [[Category: Rimlumduan T]] |
| - | [[Category: Beta-sandwich]]
| + | [[Category: Tanaka T]] |
| - | [[Category: Exoglycosidase]]
| + | |
| - | [[Category: Glycoside hydrolase]]
| + | |
| - | [[Category: Hydrolase]]
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| Structural highlights
Function
B8ANX7_ORYSI
Publication Abstract from PubMed
Most plant beta-galactosidases, which belong to glycoside hydrolase family 35, have a C-terminal domain homologous to animal galactose and rhamnose-binding lectins. To investigate the structure and function of this domain, the C-terminal domain of the rice (Oryza sativa L.) beta-galactosidase 1 (OsBGal1 Cter) was expressed in Escherichia coli and purified to homogeneity. The free OsBGal1 Cter is monomeric with a native molecular weight of 15kDa. NMR spectroscopy indicated that OsBGal1 Cter comprises five beta-strands and one alpha-helix. The structure of this domain is similar to lectin domains from animals, but loops A and C of OsBGal1 Cter are longer than the corresponding loops from related animal lectins with known structures. In addition, loop A of OsBGal1 Cter was not well defined, suggesting it is flexible. Although OsBGal1 Cter was predicted to be a galactose/rhamnose-binding domain, binding with rhamnose, galactose, glucose, beta-1,4-d-galactobiose and raffinose could not be observed in NMR experiments.
Structure of a plant beta-galactosidase C-terminal domain.,Rimlumduan T, Hua YL, Tanaka T, Ketudat Cairns JR Biochim Biophys Acta. 2016 Jul 22;1864(10):1411-1418. doi:, 10.1016/j.bbapap.2016.07.005. PMID:27451952[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Rimlumduan T, Hua YL, Tanaka T, Ketudat Cairns JR. Structure of a plant beta-galactosidase C-terminal domain. Biochim Biophys Acta. 2016 Jul 22;1864(10):1411-1418. doi:, 10.1016/j.bbapap.2016.07.005. PMID:27451952 doi:http://dx.doi.org/10.1016/j.bbapap.2016.07.005
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