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| | ==Phenol-soluble modulin Alpha 3== | | ==Phenol-soluble modulin Alpha 3== |
| - | <StructureSection load='5kgy' size='340' side='right'caption='[[5kgy]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='5kgy' size='340' side='right'caption='[[5kgy]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5kgy]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KGY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KGY FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5kgy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_RF122 Staphylococcus aureus RF122]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KGY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5KGY FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5kgz|5kgz]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5kgy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kgy OCA], [https://pdbe.org/5kgy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5kgy RCSB], [https://www.ebi.ac.uk/pdbsum/5kgy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5kgy ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kgy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kgy OCA], [http://pdbe.org/5kgy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kgy RCSB], [http://www.ebi.ac.uk/pdbsum/5kgy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kgy ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PSMA3_STAAB PSMA3_STAAB]] Peptide which can recruit, activate and subsequently lyse neutrophils, thus eliminating the main cellular defense against infection. | + | [https://www.uniprot.org/uniprot/PSMA1_STAA8 PSMA1_STAA8] Peptide which can recruit, activate and subsequently lyse human neutrophils, thus eliminating the main cellular defense against infection.[UniProtKB:A9JX05] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Acedo, J Z]] | + | [[Category: Staphylococcus aureus RF122]] |
| - | [[Category: Belkum, M J.Van]] | + | [[Category: Acedo JZ]] |
| - | [[Category: Lohans, C T]] | + | [[Category: Lohans CT]] |
| - | [[Category: Miskolzie, M]] | + | [[Category: Miskolzie M]] |
| - | [[Category: Towle, K M]] | + | [[Category: Towle KM]] |
| - | [[Category: Vederas, J C]] | + | [[Category: Van Belkum MJ]] |
| - | [[Category: Structure from cyana 2 1]] | + | [[Category: Vederas JC]] |
| Structural highlights
Function
PSMA1_STAA8 Peptide which can recruit, activate and subsequently lyse human neutrophils, thus eliminating the main cellular defense against infection.[UniProtKB:A9JX05]
Publication Abstract from PubMed
Phenol-soluble modulins (PSMs) are peptide virulence factors produced by staphylococci. These peptides contribute to the overall pathogenicity of these bacteria, eliciting multiple immune responses from host cells. Many of the alpha-type PSMs exhibit cytolytic properties and are able to lyse particular eukaryotic cells, including erythrocytes, neutrophils, and leukocytes. In addition, they also appear to contribute to the protection of the bacterial cell from the host immune response through biofilm formation and detachment. In this study, three of these peptide toxins, PSMs alpha1, alpha3, and beta2, normally produced by Staphylococcus aureus, have been synthesized using solid-supported peptide synthesis (SPPS) (PSMalpha1 and PSMalpha3) or made by heterologous expression in Escherichia coli (PSMbeta2). Their three-dimensional structures were elucidated using nuclear magnetic resonance spectroscopy. PSMalpha1 and PSMalpha3 each consist of a single amphipathic helix with a slight bend near the N- and C-termini, respectively. PSMbeta2 contains three amphipathic helices, which fold to produce a "v-like" shape between alpha-helix 2 and alpha-helix 3, with alpha-helix 1 folded over such that it is perpendicular to alpha-helix 3. The availability of three-dimensional structures permits spatial analysis of features and residues proposed to control the biological activity of these peptide toxins.
Solution Structures of Phenol-Soluble Modulins alpha1, alpha3, and beta2, Virulence Factors from Staphylococcus aureus.,Towle KM, Lohans CT, Miskolzie M, Acedo JZ, van Belkum MJ, Vederas JC Biochemistry. 2016 Aug 30;55(34):4798-806. doi: 10.1021/acs.biochem.6b00615. Epub, 2016 Aug 15. PMID:27525453[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Towle KM, Lohans CT, Miskolzie M, Acedo JZ, van Belkum MJ, Vederas JC. Solution Structures of Phenol-Soluble Modulins alpha1, alpha3, and beta2, Virulence Factors from Staphylococcus aureus. Biochemistry. 2016 Aug 30;55(34):4798-806. doi: 10.1021/acs.biochem.6b00615. Epub, 2016 Aug 15. PMID:27525453 doi:http://dx.doi.org/10.1021/acs.biochem.6b00615
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