|
|
| Line 1: |
Line 1: |
| | | | |
| | ==Solution structure of the complex between human ZNHIT3 and NUFIP1 proteins== | | ==Solution structure of the complex between human ZNHIT3 and NUFIP1 proteins== |
| - | <StructureSection load='5l85' size='340' side='right'caption='[[5l85]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='5l85' size='340' side='right'caption='[[5l85]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5l85]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5L85 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5L85 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5l85]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5L85 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5L85 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ZNHIT3, TRIP3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), NUFIP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5l85 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5l85 OCA], [https://pdbe.org/5l85 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5l85 RCSB], [https://www.ebi.ac.uk/pdbsum/5l85 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5l85 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5l85 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5l85 OCA], [http://pdbe.org/5l85 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5l85 RCSB], [http://www.ebi.ac.uk/pdbsum/5l85 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5l85 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/NUFP1_HUMAN NUFP1_HUMAN]] Binds RNA.<ref>PMID:10556305</ref> | + | [https://www.uniprot.org/uniprot/ZNHI3_HUMAN ZNHI3_HUMAN] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 22: |
Line 21: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Chagot, M E]] | + | [[Category: Chagot M-E]] |
| - | [[Category: Manival, X]] | + | [[Category: Manival X]] |
| - | [[Category: Quinternet, M]] | + | [[Category: Quinternet M]] |
| - | [[Category: Pac-hit fold jaw helice]]
| + | |
| - | [[Category: Signaling protein]]
| + | |
| Structural highlights
Function
ZNHI3_HUMAN
Publication Abstract from PubMed
Box C/D small nucleolar ribonucleoparticles (snoRNPs) support 2'-O-methylation of several target RNAs. They share a common set of four core proteins (SNU13, NOP58, NOP56, and FBL) that are assembled on different guide small nucleolar RNAs. Assembly of these entities involves additional protein factors that are absent in the mature active particle. In this context, the platform protein NUFIP1/Rsa1 establishes direct and simultaneous contacts with core proteins and with the components of the assembly machinery. Here, we solve the nuclear magnetic resonance (NMR) structure of a complex resulting from interaction between protein fragments of human NUFIP1 and its cofactor ZNHIT3, and emphasize their imbrication. Using yeast two-hybrid and complementation assays, protein co-expression, isothermal titration calorimetry, and NMR, we demonstrate that yeast and human complexes involving NUFIP1/Rsa1p, ZNHIT3/Hit1p, and SNU13/Snu13p share strong structural similarities, suggesting that the initial steps of the box C/D snoRNP assembly process are conserved among species.
Structural Features of the Box C/D snoRNP Pre-assembly Process Are Conserved through Species.,Quinternet M, Chagot ME, Rothe B, Tiotiu D, Charpentier B, Manival X Structure. 2016 Sep 1. pii: S0969-2126(16)30227-1. doi:, 10.1016/j.str.2016.07.016. PMID:27594683[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Quinternet M, Chagot ME, Rothe B, Tiotiu D, Charpentier B, Manival X. Structural Features of the Box C/D snoRNP Pre-assembly Process Are Conserved through Species. Structure. 2016 Sep 1. pii: S0969-2126(16)30227-1. doi:, 10.1016/j.str.2016.07.016. PMID:27594683 doi:http://dx.doi.org/10.1016/j.str.2016.07.016
|
