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| | ==lactococcin A immunity protein== | | ==lactococcin A immunity protein== |
| - | <StructureSection load='5lfi' size='340' side='right'caption='[[5lfi]], [[NMR_Ensembles_of_Models | 16 NMR models]]' scene=''> | + | <StructureSection load='5lfi' size='340' side='right'caption='[[5lfi]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5lfi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"lactococcus_lactis_subsp._diacitilactis_(sic)"_(garvie_and_farrow_1982)_schleifer_et_al._1986 "lactococcus lactis subsp. diacitilactis (sic)" (garvie and farrow 1982) schleifer et al. 1986]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LFI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LFI FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5lfi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactococcus_lactis_subsp._lactis Lactococcus lactis subsp. lactis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LFI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LFI FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lciA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1360 "Lactococcus lactis subsp. diacitilactis (sic)" (Garvie and Farrow 1982) Schleifer et al. 1986])</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5lfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lfi OCA], [https://pdbe.org/5lfi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5lfi RCSB], [https://www.ebi.ac.uk/pdbsum/5lfi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5lfi ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lfi OCA], [http://pdbe.org/5lfi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lfi RCSB], [http://www.ebi.ac.uk/pdbsum/5lfi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lfi ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/LCIA_LACLL LCIA_LACLL]] Imparts immunity to lactococcin-A to naturally sensitive host strains. | + | [https://www.uniprot.org/uniprot/LCIA_LACLL LCIA_LACLL] Imparts immunity to lactococcin-A to naturally sensitive host strains. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Lactococcus lactis subsp. lactis]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Fuochi, V]] | + | [[Category: Fuochi V]] |
| - | [[Category: Karlsson, B G]] | + | [[Category: Karlsson BG]] |
| - | [[Category: Kristiansen, P E]] | + | [[Category: Kristiansen PE]] |
| - | [[Category: Nissen-Meyer, J]] | + | [[Category: Nissen-Meyer J]] |
| - | [[Category: Oppegard, C]] | + | [[Category: Oppegard C]] |
| - | [[Category: Pedersen, A]] | + | [[Category: Pedersen A]] |
| - | [[Category: Persson, C]] | + | [[Category: Persson C]] |
| - | [[Category: Bacteriocin receptor]]
| + | |
| - | [[Category: Four-helix bundle]]
| + | |
| - | [[Category: Immune system]]
| + | |
| - | [[Category: Immunity protein]]
| + | |
| Structural highlights
Function
LCIA_LACLL Imparts immunity to lactococcin-A to naturally sensitive host strains.
Publication Abstract from PubMed
The class IId bacteriocin lactococcin A and the pediocin-like bacteriocins induce membrane leakage and cell death by specifically binding the mannose phophotransferase system (man-PTS) on their target cells. The bacteriocins' cognate immunity proteins that protect the producer cell from its own bacteriocin recognize and bind to the bacteriocin-man-PTS complex and thereby block membrane leakage. In this study, we have determined the three-dimensional structure of the lactococcin A immunity protein (LciA) by the use of nuclear magnetic resonance spectroscopy. LciA forms a four-helix bundle structure with a flexible C-terminal tail. Despite the low degree of sequence similarity between LciA and the pediocin-like immunity proteins, they share the same fold. However, there are certain differences between the structures. The C-terminal helix in LciA is considerably shorter than that observed in the pediocin-like immunity proteins, and the surface potentials of the immunity proteins differ. Truncated variants of LciA in which 6 or 10 of the C-terminal residues were removed yielded a reduced degree of protection, indicating that the unstructured C-terminal tail is important for the functionality of the immunity proteins.
Nuclear Magnetic Resonance Structure and Mutational Analysis of the Lactococcin A Immunity Protein.,Kristiansen PE, Persson C, Fuochi V, Pedersen A, Karlsson GB, Nissen-Meyer J, Oppegard C Biochemistry. 2016 Nov 3. PMID:27808503[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kristiansen PE, Persson C, Fuochi V, Pedersen A, Karlsson GB, Nissen-Meyer J, Oppegard C. Nuclear Magnetic Resonance Structure and Mutational Analysis of the Lactococcin A Immunity Protein. Biochemistry. 2016 Nov 3. PMID:27808503
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