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| | ==recombinant mouse Nerve Growth Factor== | | ==recombinant mouse Nerve Growth Factor== |
| - | <StructureSection load='5lsd' size='340' side='right' caption='[[5lsd]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='5lsd' size='340' side='right'caption='[[5lsd]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5lsd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LSD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LSD FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5lsd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LSD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LSD FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Ngf, Ngfb ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5lsd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lsd OCA], [https://pdbe.org/5lsd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5lsd RCSB], [https://www.ebi.ac.uk/pdbsum/5lsd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5lsd ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lsd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lsd OCA], [http://pdbe.org/5lsd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lsd RCSB], [http://www.ebi.ac.uk/pdbsum/5lsd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lsd ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/NGF_MOUSE NGF_MOUSE]] Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. | + | [https://www.uniprot.org/uniprot/NGF_MOUSE NGF_MOUSE] Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 5lsd" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 5lsd" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Nerve growth factor|Nerve growth factor]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Large Structures]] |
| - | [[Category: Cattaneo, A]] | + | [[Category: Mus musculus]] |
| - | [[Category: Chiara, C de]] | + | [[Category: Cattaneo A]] |
| - | [[Category: Kelly, G]] | + | [[Category: Kelly G]] |
| - | [[Category: Lamba, D]] | + | [[Category: Lamba D]] |
| - | [[Category: Paoletti, F]] | + | [[Category: Paoletti F]] |
| - | [[Category: Pastore, A]] | + | [[Category: Pastore A]] |
| - | [[Category: Cell cycle]] | + | [[Category: De Chiara C]] |
| - | [[Category: Cystin-knot]]
| + | |
| - | [[Category: Dimerfit_1]]
| + | |
| - | [[Category: Homodimer]]
| + | |
| - | [[Category: Ngf]]
| + | |
| Structural highlights
Function
NGF_MOUSE Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival.
Publication Abstract from PubMed
Nerve Growth Factor (NGF), the prototype of the neurotrophin family, is essential for maintenance and growth of different neuronal populations. The X-ray crystal structure of NGF has been known since the early '90s and shows a beta-sandwich fold with extensive loops that are involved in the interaction with its binding partners. Understanding the dynamical properties of these loops is thus important for molecular recognition. We present here a combined solution NMR/molecular dynamics study which addresses the question of whether and how much the long loops of NGF are flexible and describes the N-terminal intrinsic conformational tendency of the unbound NGF molecule. NMR titration experiments allowed identification of a previously undetected epitope of the anti-NGF antagonist antibody alphaD11 which will be of crucial importance for future drug lead discovery. The present study thus recapitulates all the available structural information and unveils the conformational versatility of the relatively rigid NGF loops upon functional ligand binding.
Conformational Rigidity within Plasticity Promotes Differential Target Recognition of Nerve Growth Factor.,Paoletti F, de Chiara C, Kelly G, Covaceuszach S, Malerba F, Yan R, Lamba D, Cattaneo A, Pastore A Front Mol Biosci. 2016 Dec 26;3:83. doi: 10.3389/fmolb.2016.00083. eCollection, 2016. PMID:28083536[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Paoletti F, de Chiara C, Kelly G, Covaceuszach S, Malerba F, Yan R, Lamba D, Cattaneo A, Pastore A. Conformational Rigidity within Plasticity Promotes Differential Target Recognition of Nerve Growth Factor. Front Mol Biosci. 2016 Dec 26;3:83. doi: 10.3389/fmolb.2016.00083. eCollection, 2016. PMID:28083536 doi:http://dx.doi.org/10.3389/fmolb.2016.00083
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