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| | ==Solution structure of B. subtilis Sigma G inhibitor CsfB== | | ==Solution structure of B. subtilis Sigma G inhibitor CsfB== |
| - | <StructureSection load='5n7y' size='340' side='right'caption='[[5n7y]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='5n7y' size='340' side='right'caption='[[5n7y]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5n7y]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N7Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5N7Y FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5n7y]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N7Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5N7Y FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">csfB, gin, yaaM, BSU00240 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Vibrio subtilis" Ehrenberg 1835])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5n7y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n7y OCA], [https://pdbe.org/5n7y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5n7y RCSB], [https://www.ebi.ac.uk/pdbsum/5n7y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5n7y ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5n7y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n7y OCA], [http://pdbe.org/5n7y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n7y RCSB], [http://www.ebi.ac.uk/pdbsum/5n7y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n7y ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/GIN_BACSU GIN_BACSU]] An anti-sigma-G factor, prevents premature activation of sigma-G factor in the forespore; overexpression leads to 1000-fold reduction in spore formation, spore formation stops after engulfment (PubMed:17921305, PubMed:19497328). Overexpression also inhibits sigma-G transcription activation activity (PubMed:18208527). When both Gin and sigma-G are expressed in E.coli Gin inhibits sigma-G, strongly suggesting Gin inhibits by direct physical interaction (PubMed:19497328).<ref>PMID:17921305</ref> <ref>PMID:18208527</ref> <ref>PMID:19497328</ref> | + | [https://www.uniprot.org/uniprot/GIN_BACSU GIN_BACSU] An anti-sigma-G factor, prevents premature activation of sigma-G factor in the forespore; overexpression leads to 1000-fold reduction in spore formation, spore formation stops after engulfment (PubMed:17921305, PubMed:19497328). Overexpression also inhibits sigma-G transcription activation activity (PubMed:18208527). When both Gin and sigma-G are expressed in E.coli Gin inhibits sigma-G, strongly suggesting Gin inhibits by direct physical interaction (PubMed:19497328).<ref>PMID:17921305</ref> <ref>PMID:18208527</ref> <ref>PMID:19497328</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Vibrio subtilis ehrenberg 1835]] | + | [[Category: Bacillus subtilis]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Alfano, C]] | + | [[Category: Alfano C]] |
| - | [[Category: Atkinson, A]] | + | [[Category: Atkinson A]] |
| - | [[Category: Isaacson, R L]] | + | [[Category: Isaacson RL]] |
| - | [[Category: Martinez-Lumbreras, S]] | + | [[Category: Martinez-Lumbreras S]] |
| - | [[Category: Csfb]]
| + | |
| - | [[Category: Dimer]]
| + | |
| - | [[Category: Sigma g inhibitor]]
| + | |
| - | [[Category: Transcription]]
| + | |
| - | [[Category: Zinc-finger]]
| + | |
| Structural highlights
Function
GIN_BACSU An anti-sigma-G factor, prevents premature activation of sigma-G factor in the forespore; overexpression leads to 1000-fold reduction in spore formation, spore formation stops after engulfment (PubMed:17921305, PubMed:19497328). Overexpression also inhibits sigma-G transcription activation activity (PubMed:18208527). When both Gin and sigma-G are expressed in E.coli Gin inhibits sigma-G, strongly suggesting Gin inhibits by direct physical interaction (PubMed:19497328).[1] [2] [3]
Publication Abstract from PubMed
Global changes in bacterial gene expression can be orchestrated by the coordinated activation/deactivation of alternative sigma (sigma) factor subunits of RNA polymerase. Sigma factors themselves are regulated in myriad ways, including via anti-sigma factors. Here, we have determined the solution structure of anti-sigma factor CsfB, responsible for inhibition of two alternative sigma factors, sigma(G) and sigma(E), during spore formation by Bacillus subtilis. CsfB assembles into a symmetrical homodimer, with each monomer bound to a single Zn(2+) ion via a treble-clef zinc finger fold. Directed mutagenesis indicates that dimer formation is critical for CsfB-mediated inhibition of both sigma(G) and sigma(E), and we have characterized these interactions in vitro. This work represents an advance in our understanding of how CsfB mediates inhibition of two alternative sigma factors to drive developmental gene expression in a bacterium.
Structural and Functional Insights into Bacillus subtilis Sigma Factor Inhibitor, CsfB.,Martinez-Lumbreras S, Alfano C, Evans NJ, Collins KM, Flanagan KA, Atkinson RA, Krysztofinska EM, Vydyanath A, Jackter J, Fixon-Owoo S, Camp AH, Isaacson RL Structure. 2018 Mar 3. pii: S0969-2126(18)30047-9. doi:, 10.1016/j.str.2018.02.007. PMID:29526435[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Chary VK, Xenopoulos P, Piggot PJ. Expression of the sigmaF-directed csfB locus prevents premature appearance of sigmaG activity during sporulation of Bacillus subtilis. J Bacteriol. 2007 Dec;189(23):8754-7. doi: 10.1128/JB.01265-07. Epub 2007 Oct 5. PMID:17921305 doi:http://dx.doi.org/10.1128/JB.01265-07
- ↑ Karmazyn-Campelli C, Rhayat L, Carballido-Lopez R, Duperrier S, Frandsen N, Stragier P. How the early sporulation sigma factor sigmaF delays the switch to late development in Bacillus subtilis. Mol Microbiol. 2008 Mar;67(5):1169-80. doi: 10.1111/j.1365-2958.2008.06121.x., Epub 2008 Jan 15. PMID:18208527 doi:http://dx.doi.org/10.1111/j.1365-2958.2008.06121.x
- ↑ Rhayat L, Duperrier S, Carballido-Lopez R, Pellegrini O, Stragier P. Genetic dissection of an inhibitor of the sporulation sigma factor sigma(G). J Mol Biol. 2009 Jul 31;390(5):835-44. doi: 10.1016/j.jmb.2009.05.073. Epub 2009 , Jun 1. PMID:19497328 doi:http://dx.doi.org/10.1016/j.jmb.2009.05.073
- ↑ Martinez-Lumbreras S, Alfano C, Evans NJ, Collins KM, Flanagan KA, Atkinson RA, Krysztofinska EM, Vydyanath A, Jackter J, Fixon-Owoo S, Camp AH, Isaacson RL. Structural and Functional Insights into Bacillus subtilis Sigma Factor Inhibitor, CsfB. Structure. 2018 Mar 3. pii: S0969-2126(18)30047-9. doi:, 10.1016/j.str.2018.02.007. PMID:29526435 doi:http://dx.doi.org/10.1016/j.str.2018.02.007
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