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| | ==Structure of the N-terminal domain of the Escherichia Coli ProQ RNA binding protein== | | ==Structure of the N-terminal domain of the Escherichia Coli ProQ RNA binding protein== |
| - | <StructureSection load='5nb9' size='340' side='right'caption='[[5nb9]], [[NMR_Ensembles_of_Models | 17 NMR models]]' scene=''> | + | <StructureSection load='5nb9' size='340' side='right'caption='[[5nb9]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5nb9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Eco45 Eco45]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NB9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NB9 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5nb9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_S88 Escherichia coli S88]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NB9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NB9 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">proQ, ECS88_1884 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=585035 ECO45])</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5nb9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nb9 OCA], [https://pdbe.org/5nb9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5nb9 RCSB], [https://www.ebi.ac.uk/pdbsum/5nb9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5nb9 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nb9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nb9 OCA], [http://pdbe.org/5nb9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nb9 RCSB], [http://www.ebi.ac.uk/pdbsum/5nb9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nb9 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PROQ_ECO45 PROQ_ECO45]] RNA chaperone with significant RNA binding, RNA strand exchange and RNA duplexing activities. May regulate ProP activity through an RNA-based, post-transcriptional mechanism. | + | [https://www.uniprot.org/uniprot/PROQ_ECOLI PROQ_ECOLI] RNA chaperone with significant RNA binding, RNA strand exchange and RNA duplexing activities. May regulate ProP activity through an RNA-based, post-transcriptional mechanism.[HAMAP-Rule:MF_00749]<ref>PMID:21381725</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Eco45]] | + | [[Category: Escherichia coli S88]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Bateman, A]] | + | [[Category: Bateman A]] |
| - | [[Category: Broadhurst, R]] | + | [[Category: Broadhurst R]] |
| - | [[Category: Gonzales, G]] | + | [[Category: Gonzales G]] |
| - | [[Category: Hardwick, S]] | + | [[Category: Hardwick S]] |
| - | [[Category: Holmqvist, E]] | + | [[Category: Holmqvist E]] |
| - | [[Category: Luisi, B]] | + | [[Category: Luisi B]] |
| - | [[Category: Maslen, S]] | + | [[Category: Maslen S]] |
| - | [[Category: Skehel, M]] | + | [[Category: Skehel M]] |
| - | [[Category: Vogel, J]] | + | [[Category: Vogel J]] |
| - | [[Category: Chaperone]]
| + | |
| - | [[Category: Fino]]
| + | |
| - | [[Category: Proq]]
| + | |
| - | [[Category: Rna]]
| + | |
| Structural highlights
Function
PROQ_ECOLI RNA chaperone with significant RNA binding, RNA strand exchange and RNA duplexing activities. May regulate ProP activity through an RNA-based, post-transcriptional mechanism.[HAMAP-Rule:MF_00749][1]
Publication Abstract from PubMed
The protein ProQ has recently been identified as a global small noncoding RNA-binding protein in Salmonella, and a similar role is anticipated for its numerous homologs in divergent bacterial species. We report the solution structure of Escherichia coli ProQ, revealing an N-terminal FinO-like domain, a C-terminal domain that unexpectedly has a Tudor domain fold commonly found in eukaryotes, and an elongated bridging intradomain linker that is flexible but nonetheless incompressible. Structure-based sequence analysis suggests that the Tudor domain was acquired through horizontal gene transfer and gene fusion to the ancestral FinO-like domain. Through a combination of biochemical and biophysical approaches, we have mapped putative RNA-binding surfaces on all three domains of ProQ and modeled the protein's conformation in the apo and RNA-bound forms. Taken together, these data suggest how the FinO, Tudor, and linker domains of ProQ cooperate to recognize complex RNA structures and serve to promote RNA-mediated regulation.
Structure of the Escherichia coli ProQ RNA-binding protein.,Gonzalez GM, Hardwick SW, Maslen SL, Skehel JM, Holmqvist E, Vogel J, Bateman A, Luisi BF, Broadhurst RW RNA. 2017 May;23(5):696-711. doi: 10.1261/rna.060343.116. Epub 2017 Feb 13. PMID:28193673[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Chaulk SG, Smith Frieday MN, Arthur DC, Culham DE, Edwards RA, Soo P, Frost LS, Keates RA, Glover JN, Wood JM. ProQ is an RNA chaperone that controls ProP levels in Escherichia coli. Biochemistry. 2011 Apr 19;50(15):3095-106. doi: 10.1021/bi101683a. Epub 2011 Mar , 23. PMID:21381725 doi:http://dx.doi.org/10.1021/bi101683a
- ↑ Gonzalez GM, Hardwick SW, Maslen SL, Skehel JM, Holmqvist E, Vogel J, Bateman A, Luisi BF, Broadhurst RW. Structure of the Escherichia coli ProQ RNA-binding protein. RNA. 2017 May;23(5):696-711. doi: 10.1261/rna.060343.116. Epub 2017 Feb 13. PMID:28193673 doi:http://dx.doi.org/10.1261/rna.060343.116
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