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| | ==NMR solution structure of the aromatic mutant H43W H67F cytochrome b5== | | ==NMR solution structure of the aromatic mutant H43W H67F cytochrome b5== |
| - | <StructureSection load='5xe4' size='340' side='right'caption='[[5xe4]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='5xe4' size='340' side='right'caption='[[5xe4]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5xe4]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XE4 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5XE4 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5xe4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XE4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XE4 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5xee|5xee]]</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xe4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xe4 OCA], [https://pdbe.org/5xe4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xe4 RCSB], [https://www.ebi.ac.uk/pdbsum/5xe4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xe4 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5xe4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xe4 OCA], [http://pdbe.org/5xe4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xe4 RCSB], [http://www.ebi.ac.uk/pdbsum/5xe4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xe4 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CYB5_RAT CYB5_RAT]] Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. It is also involved in several steps of the sterol biosynthesis pathway, particularly in the C-6 double bond introduction during the C-6 desaturation. | + | [https://www.uniprot.org/uniprot/CYB5_RAT CYB5_RAT] Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. It is also involved in several steps of the sterol biosynthesis pathway, particularly in the C-6 double bond introduction during the C-6 desaturation. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Balakrishnan, S]] | + | [[Category: Rattus norvegicus]] |
| - | [[Category: Sarma, S P]] | + | [[Category: Balakrishnan S]] |
| - | [[Category: Aromatic interactions protein folding]] | + | [[Category: Sarma SP]] |
| - | [[Category: Electron transport]]
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| Structural highlights
Function
CYB5_RAT Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. It is also involved in several steps of the sterol biosynthesis pathway, particularly in the C-6 double bond introduction during the C-6 desaturation.
Publication Abstract from PubMed
Aromatic interactions are an important force in protein folding as they combine the stability of a hydrophobic interaction with the selectivity of a hydrogen bond. Much of our understanding of aromatic interactions comes from "bioinformatics" based analyses of protein structures and from the contribution of these interactions to stabilizing secondary structure motifs in model peptides. In this study, the structural consequences of aromatic interactions on protein folding have been explored in engineered mutants of the molten globule protein apo-cytochrome b5. Structural changes from disorder to order due to aromatic interactions in two variants of the protein, viz., WF-cytb5 and FF-cytb5, result in significant long-range secondary and tertiary structure. The results show that 54 and 52% of the residues in WF-cytb5 and FF-cytb5, respectively, occupy ordered regions versus 26% in apo-cytochrome b5. The interactions between the aromatic groups are offset-stacked and edge-to-face for the Trp-Phe and Phe-Phe mutants, respectively. Urea denaturation studies indicate that both mutants have a Cm higher than that of apo-cytochrome b5 and are more stable to chaotropic agents than apo-cytochrome b5. The introduction of these aromatic residues also results in "trimer" interactions with existing aromatic groups, reaffirming the selectivity of the aromatic interactions. These studies provide insights into the aromatic interactions that drive disorder-to-order transitions in intrinsically disordered regions of proteins and will aid in de novo protein design beyond small peptide scaffolds.
Engineering Aromatic-Aromatic Interactions To Nucleate Folding in Intrinsically Disordered Regions of Proteins.,Balakrishnan S, Sarma SP Biochemistry. 2017 Aug 22;56(33):4346-4359. doi: 10.1021/acs.biochem.7b00437., Epub 2017 Aug 11. PMID:28738155[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Balakrishnan S, Sarma SP. Engineering Aromatic-Aromatic Interactions To Nucleate Folding in Intrinsically Disordered Regions of Proteins. Biochemistry. 2017 Aug 22;56(33):4346-4359. doi: 10.1021/acs.biochem.7b00437., Epub 2017 Aug 11. PMID:28738155 doi:http://dx.doi.org/10.1021/acs.biochem.7b00437
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