|
|
| Line 1: |
Line 1: |
| | | | |
| | ==Solution NMR structure of a new lasso peptide specialicin== | | ==Solution NMR structure of a new lasso peptide specialicin== |
| - | <StructureSection load='6ak0' size='340' side='right'caption='[[6ak0]], [[NMR_Ensembles_of_Models | 15 NMR models]]' scene=''> | + | <StructureSection load='6ak0' size='340' side='right'caption='[[6ak0]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6ak0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_specialis Streptomyces specialis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AK0 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6AK0 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6ak0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_specialis Streptomyces specialis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AK0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6AK0 FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DTR:D-TRYPTOPHAN'>DTR</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DTR:D-TRYPTOPHAN'>DTR</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6ak0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ak0 OCA], [http://pdbe.org/6ak0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ak0 RCSB], [http://www.ebi.ac.uk/pdbsum/6ak0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ak0 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ak0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ak0 OCA], [https://pdbe.org/6ak0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ak0 RCSB], [https://www.ebi.ac.uk/pdbsum/6ak0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ak0 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0A493R6M6_9ACTN A0A493R6M6_9ACTN] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 22: |
Line 24: |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| | [[Category: Streptomyces specialis]] | | [[Category: Streptomyces specialis]] |
| - | [[Category: Hemmi, H]] | + | [[Category: Hemmi H]] |
| - | [[Category: Kaweewan, I]] | + | [[Category: Kaweewan I]] |
| - | [[Category: Kodani, S]] | + | [[Category: Kodani S]] |
| - | [[Category: Komaki, H]] | + | [[Category: Komaki H]] |
| - | [[Category: Lasso peptide]]
| + | |
| - | [[Category: Specialicin]]
| + | |
| - | [[Category: Streptomyces speciali]]
| + | |
| - | [[Category: Unknown function]]
| + | |
| Structural highlights
Function
A0A493R6M6_9ACTN
Publication Abstract from PubMed
Based on genome mining, a new lasso peptide specialicin was isolated from the extract of Streptomyces specialis. The structure of specialicin was established by ESI-MS and NMR analyses to be a lasso peptide with the length of 21 amino acids, containing an isopeptide bond and two disulfide bonds in the molecule. The stereochemistries of the constituent amino acids except for Trp were determined to be L and the stereochemistry of Trp at C-terminus was determined to be D. Three dimensional structure of specialicin was determined based on NOE experimental data, which indicated that specialicin possessed the similar conformational structure with siamycin I. Specialicin showed the antibacterial activity against Micrococcus luteus and the moderate anti-HIV activity against HIV-1 NL4-3. The biosynthetic gene cluster of specialicin was proposed from the genome sequence data of S. specialis.
Isolation and structure determination of a new lasso peptide specialicin based on genome mining.,Kaweewan I, Hemmi H, Komaki H, Harada S, Kodani S Bioorg Med Chem. 2018 Nov 9. pii: S0968-0896(18)31650-X. doi:, 10.1016/j.bmc.2018.11.007. PMID:30448257[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kaweewan I, Hemmi H, Komaki H, Harada S, Kodani S. Isolation and structure determination of a new lasso peptide specialicin based on genome mining. Bioorg Med Chem. 2018 Nov 9. pii: S0968-0896(18)31650-X. doi:, 10.1016/j.bmc.2018.11.007. PMID:30448257 doi:http://dx.doi.org/10.1016/j.bmc.2018.11.007
|
