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| | ==NMR Structure of Salmonella Type III Secretion system protein OrgC== | | ==NMR Structure of Salmonella Type III Secretion system protein OrgC== |
| - | <StructureSection load='6cjd' size='340' side='right'caption='[[6cjd]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='6cjd' size='340' side='right'caption='[[6cjd]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6cjd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Salet Salet]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CJD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CJD FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6cjd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica Salmonella enterica subsp. enterica]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CJD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6CJD FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">orgC, CVR97_12460, SE14_03054 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=59201 SALET])</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6cjd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cjd OCA], [https://pdbe.org/6cjd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6cjd RCSB], [https://www.ebi.ac.uk/pdbsum/6cjd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6cjd ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cjd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cjd OCA], [http://pdbe.org/6cjd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cjd RCSB], [http://www.ebi.ac.uk/pdbsum/6cjd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cjd ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0A0H3NF83_SALTS A0A0H3NF83_SALTS] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Salet]] | + | [[Category: Salmonella enterica subsp. enterica]] |
| - | [[Category: DEY, S]]
| + | [[Category: DE GUZMAN RN]] |
| - | [[Category: GUZMAN, R N.DE]] | + | [[Category: DEY S]] |
| - | [[Category: Controls needle assembly of t3ss]] | + | |
| - | [[Category: Early substrate of t3ss]]
| + | |
| - | [[Category: Protein binding]]
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| Structural highlights
Function
A0A0H3NF83_SALTS
Publication Abstract from PubMed
Type III protein secretion systems (T3SS) are encoded by several pathogenic or symbiotic bacteria. The central component of this nanomachine is the needle complex. Here we show in a Salmonella Typhimurium T3SS that assembly of the needle filament of this structure requires OrgC, a protein encoded within the T3SS gene cluster. Absence of OrgC results in significantly reduced number of needle substructures but does not affect needle length. We show that OrgC is secreted by the T3SS and that exogenous addition of OrgC can complement a orgC mutation. We also show that OrgC interacts with the needle filament subunit PrgI and accelerates its polymerization into filaments in vitro. The structure of OrgC shows a novel fold with a shared topology with a domain from flagellar capping proteins. These findings identify a novel component of T3SS and provide new insight into the assembly of the type III secretion machine.
A protein secreted by the Salmonella type III secretion system controls needle filament assembly.,Kato J, Dey S, Soto JE, Butan C, Wilkinson MC, De Guzman RN, Galan JE Elife. 2018 Jul 17;7. pii: 35886. doi: 10.7554/eLife.35886. PMID:30015613[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kato J, Dey S, Soto JE, Butan C, Wilkinson MC, De Guzman RN, Galan JE. A protein secreted by the Salmonella type III secretion system controls needle filament assembly. Elife. 2018 Jul 17;7. pii: 35886. doi: 10.7554/eLife.35886. PMID:30015613 doi:http://dx.doi.org/10.7554/eLife.35886
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