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| | ==Solution structure of Rbfox2 RRM mimetic peptide CPfox7== | | ==Solution structure of Rbfox2 RRM mimetic peptide CPfox7== |
| - | <StructureSection load='6dze' size='340' side='right'caption='[[6dze]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | + | <StructureSection load='6dze' size='340' side='right'caption='[[6dze]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6dze]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DZE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DZE FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6dze]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_fischeri Aspergillus fischeri]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DZE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6DZE FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DPR:D-PROLINE'>DPR</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DPR:D-PROLINE'>DPR</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6dze FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dze OCA], [http://pdbe.org/6dze PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dze RCSB], [http://www.ebi.ac.uk/pdbsum/6dze PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dze ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6dze FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dze OCA], [https://pdbe.org/6dze PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6dze RCSB], [https://www.ebi.ac.uk/pdbsum/6dze PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6dze ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Aspergillus fischeri]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Sun, Y T]] | + | [[Category: Sun Y-T]] |
| - | [[Category: Varani, G]] | + | [[Category: Varani G]] |
| - | [[Category: Peptide structure]]
| + | |
| - | [[Category: Peptidomimetic]]
| + | |
| - | [[Category: Rna binding protein]]
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| - | [[Category: Rna recognition]]
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| Structural highlights
Publication Abstract from PubMed
The RNA recognition motif (RRM), which is the most abundant RNA-binding motif in eukaryotes, is a well-structured domain of about 90 amino acids, yet the beta2beta3 hairpin, corresponding to strands 2 and 3 of the beta-sheet, and the intervening loop make essential interactions with RNA in many RRM complexes. A series of small cyclic peptide mimics of the beta2beta3 hairpin of Rbfox2 protein that recognize the terminal loop of precursor miR-20b have been designed to investigate whether the full RNA-binding protein can be mimicked with a minimal structurally preorganized peptide. Within a small library of seven cyclic peptides, a peptide with low-micromolar affinity for the miR-20b precursor was found. NMR spectroscopy titration data suggest that this peptide specifically targets the apical loop of pre-miR-20b. This work shows that it is possible to mimic RNA-binding proteins with designed stable peptides, which provide a starting point for designing or evolving small peptide mimetics of RRM proteins.
A Small Cyclic beta-Hairpin Peptide Mimics the Rbfox2 RNA Recognition Motif and Binds to the Precursor miRNA 20b.,Sun YT, Shortridge MD, Varani G Chembiochem. 2019 Apr 1;20(7):931-939. doi: 10.1002/cbic.201800645. Epub 2019 Feb, 15. PMID:30537200[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Sun YT, Shortridge MD, Varani G. A Small Cyclic beta-Hairpin Peptide Mimics the Rbfox2 RNA Recognition Motif and Binds to the Precursor miRNA 20b. Chembiochem. 2019 Apr 1;20(7):931-939. doi: 10.1002/cbic.201800645. Epub 2019 Feb, 15. PMID:30537200 doi:http://dx.doi.org/10.1002/cbic.201800645
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