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| | ==Solution structure of the TPR domain of the cell division coordinator, CpoB== | | ==Solution structure of the TPR domain of the cell division coordinator, CpoB== |
| - | <StructureSection load='6g5s' size='340' side='right'caption='[[6g5s]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='6g5s' size='340' side='right'caption='[[6g5s]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6g5s]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6G5S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6G5S FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6g5s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6G5S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6G5S FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2xev|2xev]]</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6g5s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6g5s OCA], [https://pdbe.org/6g5s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6g5s RCSB], [https://www.ebi.ac.uk/pdbsum/6g5s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6g5s ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cpoB, ybgF, b0742, JW0732 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6g5s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6g5s OCA], [http://pdbe.org/6g5s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6g5s RCSB], [http://www.ebi.ac.uk/pdbsum/6g5s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6g5s ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CPOB_ECOLI CPOB_ECOLI]] Mediates coordination of peptidoglycan synthesis and outer membrane constriction during cell division. Promotes physical and functional coordination of the PBP1B-LpoB and Tol machines, and regulates PBP1B activity in response to Tol energy state.<ref>PMID:25951518</ref> | + | [https://www.uniprot.org/uniprot/CPOB_ECOLI CPOB_ECOLI] Mediates coordination of peptidoglycan synthesis and outer membrane constriction during cell division. Promotes physical and functional coordination of the PBP1B-LpoB and Tol machines, and regulates PBP1B activity in response to Tol energy state.<ref>PMID:25951518</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Ecoli]] | + | [[Category: Escherichia coli K-12]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Bougault, C]] | + | [[Category: Bougault C]] |
| - | [[Category: Egan, A]] | + | [[Category: Egan A]] |
| - | [[Category: Martinez, R C.Maya]] | + | [[Category: Maya Martinez RC]] |
| - | [[Category: Simorre, J P]] | + | [[Category: Simorre JP]] |
| - | [[Category: Vollmer, W]] | + | [[Category: Vollmer W]] |
| - | [[Category: Cell cycle]]
| + | |
| - | [[Category: Cell division coordinator]]
| + | |
| - | [[Category: E. coli]]
| + | |
| - | [[Category: Pbp1b]]
| + | |
| - | [[Category: Transpeptidase regulator]]
| + | |
| Structural highlights
Function
CPOB_ECOLI Mediates coordination of peptidoglycan synthesis and outer membrane constriction during cell division. Promotes physical and functional coordination of the PBP1B-LpoB and Tol machines, and regulates PBP1B activity in response to Tol energy state.[1]
Publication Abstract from PubMed
Bacteria surround their cytoplasmic membrane with an essential, stress-bearing peptidoglycan (PG) layer consisting of glycan chains linked by short peptides into a mesh-like structure. Growing and dividing cells expand their PG layer using inner-membrane anchored PG synthases, including Penicillin-binding proteins (PBPs), which participate in dynamic protein complexes to facilitate cell wall growth. In Escherichia coli, and presumably other Gram-negative bacteria, growth of the mainly single layered PG is regulated by outer membrane-anchored lipoproteins. The lipoprotein LpoB is required to activate PBP1B, which is a major, bi-functional PG synthase with glycan chain polymerizing (glycosyltransferase) and peptide cross-linking (transpeptidase) activities. In this work we show how the binding of LpoB to the regulatory UB2H domain of PBP1B activates both activities. Binding induces structural changes in the UB2H domain, which transduce to the two catalytic domains by distinct allosteric pathways. We also show how an additional regulator protein, CpoB, is able to selectively modulate the TPase activation by LpoB without interfering with GTase activation. This article is protected by copyright. All rights reserved.
Induced conformational changes activate the peptidoglycan synthase PBP1B.,Egan AJF, Maya-Martinez R, Ayala I, Bougault CM, Banzhaf M, Breukink E, Vollmer W, Simorre JP Mol Microbiol. 2018 Jul 25. doi: 10.1111/mmi.14082. PMID:30044025[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Gray AN, Egan AJ, Van't Veer IL, Verheul J, Colavin A, Koumoutsi A, Biboy J, Altelaar AF, Damen MJ, Huang KC, Simorre JP, Breukink E, den Blaauwen T, Typas A, Gross CA, Vollmer W. Coordination of peptidoglycan synthesis and outer membrane constriction during Escherichia coli cell division. Elife. 2015 May 7;4. doi: 10.7554/eLife.07118. PMID:25951518 doi:http://dx.doi.org/10.7554/eLife.07118
- ↑ Egan AJF, Maya-Martinez R, Ayala I, Bougault CM, Banzhaf M, Breukink E, Vollmer W, Simorre JP. Induced conformational changes activate the peptidoglycan synthase PBP1B. Mol Microbiol. 2018 Jul 25. doi: 10.1111/mmi.14082. PMID:30044025 doi:http://dx.doi.org/10.1111/mmi.14082
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