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| | ==Solution NMR structure of the major type IV pilin PpdD from enterohemorrhagic Escherichia coli (EHEC)== | | ==Solution NMR structure of the major type IV pilin PpdD from enterohemorrhagic Escherichia coli (EHEC)== |
| - | <StructureSection load='6gms' size='340' side='right'caption='[[6gms]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='6gms' size='340' side='right'caption='[[6gms]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6gms]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Eco57 Eco57]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GMS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GMS FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6gms]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GMS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6GMS FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ECs0112 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57])</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6gms FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gms OCA], [https://pdbe.org/6gms PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6gms RCSB], [https://www.ebi.ac.uk/pdbsum/6gms PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6gms ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gms FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gms OCA], [http://pdbe.org/6gms PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gms RCSB], [http://www.ebi.ac.uk/pdbsum/6gms PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gms ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q8X974_ECO57 Q8X974_ECO57] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Eco57]] | + | [[Category: Escherichia coli O157:H7]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Amorim, G C]] | + | [[Category: Amorim GC]] |
| - | [[Category: Bardiaux, B]] | + | [[Category: Bardiaux B]] |
| - | [[Category: Egelman, E]] | + | [[Category: Egelman E]] |
| - | [[Category: Francetic, O]] | + | [[Category: Francetic O]] |
| - | [[Category: Guilvout, I]] | + | [[Category: Guilvout I]] |
| - | [[Category: Izadi-Pruneyre, N]] | + | [[Category: Izadi-Pruneyre N]] |
| - | [[Category: Luna-Rico, A]] | + | [[Category: Luna-Rico A]] |
| - | [[Category: Nilges, M]] | + | [[Category: Nilges M]] |
| - | [[Category: Zeng, W]] | + | [[Category: Zeng W]] |
| - | [[Category: Cell adhesion]]
| + | |
| - | [[Category: Type iv pilin adhesion ehec t4p]]
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| Structural highlights
Function
Q8X974_ECO57
Publication Abstract from PubMed
Bacterial type 4a pili are dynamic surface filaments that promote bacterial adherence, motility, and macromolecular transport. Their genes are highly conserved among enterobacteria and their expression in enterohemorrhagic Escherichia coli (EHEC) promotes adhesion to intestinal epithelia and pro-inflammatory signaling. To define the molecular basis of EHEC pilus assembly, we determined the structure of the periplasmic domain of its major subunit PpdD (PpdDp), a prototype of an enterobacterial pilin subfamily containing two disulfide bonds. The structure of PpdDp, determined by NMR, was then docked into the density envelope of purified EHEC pili obtained by cryoelectron microscopy (cryo-EM). Cryo-EM reconstruction of EHEC pili at approximately 8 A resolution revealed extremely high pilus flexibility correlating with a large extended region of the pilin stem. Systematic mutagenesis combined with functional and interaction analyses identified charged residues essential for pilus assembly. Structural information on exposed regions and interfaces between EHEC pilins is relevant for vaccine and drug discovery.
Structure and Assembly of the Enterohemorrhagic Escherichia coli Type 4 Pilus.,Bardiaux B, de Amorim GC, Luna Rico A, Zheng W, Guilvout I, Jollivet C, Nilges M, Egelman EH, Izadi-Pruneyre N, Francetic O Structure. 2019 Apr 9. pii: S0969-2126(19)30117-0. doi:, 10.1016/j.str.2019.03.021. PMID:31056419[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Bardiaux B, de Amorim GC, Luna Rico A, Zheng W, Guilvout I, Jollivet C, Nilges M, Egelman EH, Izadi-Pruneyre N, Francetic O. Structure and Assembly of the Enterohemorrhagic Escherichia coli Type 4 Pilus. Structure. 2019 Apr 9. pii: S0969-2126(19)30117-0. doi:, 10.1016/j.str.2019.03.021. PMID:31056419 doi:http://dx.doi.org/10.1016/j.str.2019.03.021
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