6gvq

From Proteopedia

(Difference between revisions)

Revision as of 10:49, 14 June 2023

DNA-bound pRN1 helix bundle domain with ATP and magnesium in the interaction buffer

Structural highlights

6gvq is a 2 chain structure with sequence from Sulfolobus islandicus. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q54324_SULIS

Publication Abstract from PubMed

Primases have a fundamental role in DNA replication. They synthesize a primer that is then extended by DNA polymerases. Archaeoeukaryotic primases require for synthesis a catalytic and an accessory domain, the exact contribution of the latter being unresolved. For the pRN1 archaeal primase, this domain is a 115-amino acid helix bundle domain (HBD). Our structural investigations of this small HBD by liquid- and solid-state nuclear magnetic resonance (NMR) revealed that only the HBD binds the DNA template. DNA binding becomes sequence-specific after a major allosteric change in the HBD, triggered by the binding of two nucleotide triphosphates. The spatial proximity of the two nucleotides and the DNA template in the quaternary structure of the HBD strongly suggests that this small domain brings together the substrates to prepare the first catalytic step of primer synthesis. This efficient mechanism is likely general for all archaeoeukaryotic primases.

A Small Helical Bundle Prepares Primer Synthesis by Binding Two Nucleotides that Enhance Sequence-Specific Recognition of the DNA Template.,Boudet J, Devillier JC, Wiegand T, Salmon L, Meier BH, Lipps G, Allain FH Cell. 2018 Dec 18. pii: S0092-8674(18)31556-3. doi: 10.1016/j.cell.2018.11.031. PMID:30595448^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Boudet J, Devillier JC, Wiegand T, Salmon L, Meier BH, Lipps G, Allain FH. A Small Helical Bundle Prepares Primer Synthesis by Binding Two Nucleotides that Enhance Sequence-Specific Recognition of the DNA Template. Cell. 2018 Dec 18. pii: S0092-8674(18)31556-3. doi: 10.1016/j.cell.2018.11.031. PMID:30595448 doi:http://dx.doi.org/10.1016/j.cell.2018.11.031

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6gvq"

Categories: Large Structures | Sulfolobus islandicus | Allain FH-T | Boudet J | Lipps G

@@ Line 1: / Line 1: @@
 ==DNA-bound pRN1 helix bundle domain with ATP and magnesium in the interaction buffer==
-<StructureSection load='6gvq' size='340' side='right'caption='[[6gvq]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='6gvq' size='340' side='right'caption='[[6gvq]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6gvq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"sulfolobus_islandicus"_zillig_et_al._1994 "sulfolobus islandicus" zillig et al. 1994]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GVQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GVQ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6gvq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfolobus_islandicus Sulfolobus islandicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GVQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6GVQ FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gvq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gvq OCA], [http://pdbe.org/6gvq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gvq RCSB], [http://www.ebi.ac.uk/pdbsum/6gvq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gvq ProSAT]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6gvq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gvq OCA], [https://pdbe.org/6gvq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6gvq RCSB], [https://www.ebi.ac.uk/pdbsum/6gvq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6gvq ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q54324_SULIS Q54324_SULIS]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 15: / Line 17: @@
 </div>
 <div class="pdbe-citations 6gvq" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Sulfolobus islandicus zillig et al. 1994]]
 [[Category: Large Structures]]
-[[Category: Allain, F H.T]]
+[[Category: Sulfolobus islandicus]]
-[[Category: Boudet, J]]
+[[Category: Allain FH-T]]
-[[Category: Lipps, G]]
+[[Category: Boudet J]]
-[[Category: Dinucleotide formation]]
+[[Category: Lipps G]]
-[[Category: Dna binding protein]]
-[[Category: Prn1 primase]]
-[[Category: Quaternary structure of the helix bundle domain]]
-[[Category: Synergistic effect]]

6gvq

From Proteopedia

Revision as of 10:49, 14 June 2023

DNA-bound pRN1 helix bundle domain with ATP and magnesium in the interaction buffer

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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