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| | ==Nicomicin-1 -- Novel antimicrobial peptides from the Arctic polychaeta Nicomache minor provide new molecular insight into biological role of the BRICHOS domain== | | ==Nicomicin-1 -- Novel antimicrobial peptides from the Arctic polychaeta Nicomache minor provide new molecular insight into biological role of the BRICHOS domain== |
| - | <StructureSection load='6hn9' size='340' side='right'caption='[[6hn9]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='6hn9' size='340' side='right'caption='[[6hn9]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6hn9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Nicomache_minor Nicomache minor]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HN9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HN9 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6hn9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Nicomache_minor Nicomache minor]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HN9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6HN9 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hn9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hn9 OCA], [http://pdbe.org/6hn9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hn9 RCSB], [http://www.ebi.ac.uk/pdbsum/6hn9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hn9 ProSAT]</span></td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6hn9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hn9 OCA], [https://pdbe.org/6hn9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6hn9 RCSB], [https://www.ebi.ac.uk/pdbsum/6hn9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6hn9 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0A3G2WH77_9ANNE A0A3G2WH77_9ANNE] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| | [[Category: Nicomache minor]] | | [[Category: Nicomache minor]] |
| - | [[Category: Bolosov, I A]] | + | [[Category: Bolosov IA]] |
| - | [[Category: Marggraf, M B]] | + | [[Category: Marggraf MB]] |
| - | [[Category: Ovchinnikova, T V]] | + | [[Category: Ovchinnikova TV]] |
| - | [[Category: Panteleev, P V]] | + | [[Category: Panteleev PV]] |
| - | [[Category: Paramonov, A S]] | + | [[Category: Paramonov AS]] |
| - | [[Category: Shenkarev, Z O]] | + | [[Category: Shenkarev ZO]] |
| - | [[Category: Sychev, S V]] | + | [[Category: Sychev SV]] |
| - | [[Category: Tsarev, A V]] | + | [[Category: Tsarev AV]] |
| - | [[Category: Antimicrobial protein]]
| + | |
| - | [[Category: Protein]]
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| Structural highlights
Function
A0A3G2WH77_9ANNE
Publication Abstract from PubMed
Endogenous antimicrobial peptides (AMPs) are among the earliest molecular factors in the evolution of animal innate immunity. In this study, novel AMPs named nicomicins were identified in the small marine polychaeta Nicomache minor in the Maldanidae family. Full-length mRNA sequences encoded 239-residue prepropeptides consisting of a putative signal sequence region, the BRICHOS domain within an acidic proregion, and 33-residue mature cationic peptides. Nicomicin-1 was expressed in the bacterial system, and its spatial structure was analyzed by circular dichroism and nuclear magnetic resonance spectroscopy. Nicomicins are unique among polychaeta AMPs scaffolds, combining an amphipathic N-terminal alpha-helix and C-terminal extended part with a six-residue loop stabilized by a disulfide bridge. This structural arrangement resembles the Rana-box motif observed in the alpha-helical host-defense peptides isolated from frog skin. Nicomicin-1 exhibited strong in vitro antimicrobial activity against Gram-positive bacteria at submicromolar concentrations. The main mechanism of nicomicin-1 action is based on membrane damage but not on the inhibition of bacterial translation. The peptide possessed cytotoxicity against cancer and normal adherent cells as well as toward human erythrocytes.
Novel Antimicrobial Peptides from the Arctic Polychaeta Nicomache minor Provide New Molecular Insight into Biological Role of the BRICHOS Domain.,Panteleev PV, Tsarev AV, Bolosov IA, Paramonov AS, Marggraf MB, Sychev SV, Shenkarev ZO, Ovchinnikova TV Mar Drugs. 2018 Oct 23;16(11). pii: md16110401. doi: 10.3390/md16110401. PMID:30360541[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Panteleev PV, Tsarev AV, Bolosov IA, Paramonov AS, Marggraf MB, Sychev SV, Shenkarev ZO, Ovchinnikova TV. Novel Antimicrobial Peptides from the Arctic Polychaeta Nicomache minor Provide New Molecular Insight into Biological Role of the BRICHOS Domain. Mar Drugs. 2018 Oct 23;16(11). pii: md16110401. doi: 10.3390/md16110401. PMID:30360541 doi:http://dx.doi.org/10.3390/md16110401
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