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| | ==SOLUTION STRUCTURE OF CN29, A TOXIN FROM CENTRUROIDES NOXIUS SCORPION VENOM== | | ==SOLUTION STRUCTURE OF CN29, A TOXIN FROM CENTRUROIDES NOXIUS SCORPION VENOM== |
| - | <StructureSection load='6nw8' size='340' side='right'caption='[[6nw8]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | + | <StructureSection load='6nw8' size='340' side='right'caption='[[6nw8]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6nw8]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NW8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NW8 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6nw8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Centruroides_noxius Centruroides noxius]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NW8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NW8 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6nw8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nw8 OCA], [http://pdbe.org/6nw8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nw8 RCSB], [http://www.ebi.ac.uk/pdbsum/6nw8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nw8 ProSAT]</span></td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6nw8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nw8 OCA], [https://pdbe.org/6nw8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6nw8 RCSB], [https://www.ebi.ac.uk/pdbsum/6nw8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6nw8 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/SCX29_CENNO SCX29_CENNO] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Centruroides noxius]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Delepierre, M]] | + | [[Category: Delepierre M]] |
| - | [[Category: Guijarro, J I]] | + | [[Category: Guijarro JI]] |
| - | [[Category: Gurrola, G B]] | + | [[Category: Gurrola GB]] |
| - | [[Category: Possani, L D]] | + | [[Category: Possani LD]] |
| - | [[Category: Scorpion toxin]]
| + | |
| - | [[Category: Toxin]]
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| Structural highlights
Function
SCX29_CENNO
Publication Abstract from PubMed
A peptide (Cn29) from the venom of the scorpion Centruroides noxius (about 2% of the soluble venom) was purified and its primary and three-dimensional structures were determined. The peptide contains 27 amino acids with primary sequence: LCLSCRGGDYDCRVKGTCENGKCVCGS. The peptide is tightly packed by three disulfide linkages formed between C2-C23, C5-C18 and C12-C25. Since the native peptide was obtained in limited amounts, the full synthetic peptide was prepared using the standard F-moc-based solid phase synthesis method of Merrifield. The native and synthetic peptides were shown to be identical by sequencing, HPLC separation and mass spectrometry. The solution structure of the peptide solved from NMR data shows that it consists of a well-defined N-terminal region without regular secondary structure extending from Leu 1 to Asp 9, followed by a short helical fragment from Tyr10 to Val14 and two short beta strands (Thr17-Glu19 and Lys22-Val24). The primary and tertiary structures of Cn29 are different from all other scorpion peptides described in the literature. Transcriptome analysis of RNA obtained from C. noxius confirmed the expression of a gene coding for Cn29 in its venom gland. Initial experiments were conducted to identify its possible function: lethality tests in mice and insects as well as ion-channel binding using in vitro electrophysiological assays. None of the physiological or biological tests displayed any activity for this peptide, which at present is considered to be another orphan peptide found in scorpion venoms. The peptide is thus the first example of a novel structural component present in scorpion venoms.
Cn29, a novel orphan peptide found in the venom of the scorpion Centruroides noxius: Structure and function.,Gurrola GB, Guijarro JI, Delepierre M, Mendoza RLL, Cid-Uribe JI, Coronas FV, Possani LD Toxicon. 2019 Jun 18. pii: S0041-0101(19)30188-6. doi:, 10.1016/j.toxicon.2019.06.013. PMID:31226259[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Gurrola GB, Guijarro JI, Delepierre M, Mendoza RLL, Cid-Uribe JI, Coronas FV, Possani LD. Cn29, a novel orphan peptide found in the venom of the scorpion Centruroides noxius: Structure and function. Toxicon. 2019 Jun 18. pii: S0041-0101(19)30188-6. doi:, 10.1016/j.toxicon.2019.06.013. PMID:31226259 doi:http://dx.doi.org/10.1016/j.toxicon.2019.06.013
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