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| | ==A9 peptide derived from Herceptin fab binding region== | | ==A9 peptide derived from Herceptin fab binding region== |
| - | <StructureSection load='6s0n' size='340' side='right'caption='[[6s0n]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='6s0n' size='340' side='right'caption='[[6s0n]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6s0n]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S0N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6S0N FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6s0n]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S0N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6S0N FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6s0n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s0n OCA], [http://pdbe.org/6s0n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6s0n RCSB], [http://www.ebi.ac.uk/pdbsum/6s0n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6s0n ProSAT]</span></td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6s0n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s0n OCA], [https://pdbe.org/6s0n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6s0n RCSB], [https://www.ebi.ac.uk/pdbsum/6s0n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6s0n ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Diana, D]] | + | [[Category: De Luca S]] |
| - | [[Category: Fattorusso, R]] | + | [[Category: Diana D]] |
| - | [[Category: Luca, S De]] | + | [[Category: Fattorusso R]] |
| - | [[Category: Saviano, M]] | + | [[Category: Saviano M]] |
| - | [[Category: Verdoliva, V]] | + | [[Category: Verdoliva V]] |
| - | [[Category: Her2 receptor]]
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| - | [[Category: Peptide binding protein]]
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| - | [[Category: Peptide-protein interaction]]
| + | |
| - | [[Category: Saturation transfer difference]]
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| - | [[Category: Spr technique]]
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| Structural highlights
Publication Abstract from PubMed
The binding process of A9 peptide toward HER2-DIVMP, a synthetic model of the receptor domain IV, was studied by using the surface plasmon resonance (SPR) technique, with the aim of validating it as a fast and reliable screening method for selecting peptide ligands specifically targeting a domain of their target. To investigate the structural basis of A9 binding to the model of HER2-DIVMP, multiple ligand-based nuclear magnetic resonance (NMR) methods were applied. The use of saturation transfer difference (STD) and WaterLOGSY NMR experiments identified key residues in the peptide for the receptor binding. Moreover, the bound conformation of the A9 peptide was obtained using transferred nuclear Overhauser effect spectroscopy (trNOESY) experiments. The NMR data revealed an extended binding surface that confirms an in silico model previously reported. These structural findings could provide good starting points for future lead structures optimization specific for the receptor target.
SPR and NMR characterization of the molecular interaction between A9 peptide and a model system of HER2 receptor: A fragment approach for selecting peptide structures specific for their target.,De Luca S, Verdoliva V, Saviano M, Fattorusso R, Diana D J Pept Sci. 2019 Nov 20:e3231. doi: 10.1002/psc.3231. PMID:31749266[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ De Luca S, Verdoliva V, Saviano M, Fattorusso R, Diana D. SPR and NMR characterization of the molecular interaction between A9 peptide and a model system of HER2 receptor: A fragment approach for selecting peptide structures specific for their target. J Pept Sci. 2019 Nov 20:e3231. doi: 10.1002/psc.3231. PMID:31749266 doi:http://dx.doi.org/10.1002/psc.3231
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