6u7r
From Proteopedia
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<StructureSection load='6u7r' size='340' side='right'caption='[[6u7r]]' scene=''> | <StructureSection load='6u7r' size='340' side='right'caption='[[6u7r]]' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full | + | <table><tr><td colspan='2'>[[6u7r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Helianthus_annuus Helianthus annuus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U7R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6U7R FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6u7r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u7r OCA], [https://pdbe.org/6u7r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6u7r RCSB], [https://www.ebi.ac.uk/pdbsum/6u7r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6u7r ProSAT]</span></td></tr> |
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/SFTI1_HELAN SFTI1_HELAN] Inhibits trypsin, cathepsin G, elastase, chymotrypsin and thrombin. Does not inhibit factor Xa.<ref>PMID:10390350</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Ruthenium-catalysed azide-alkyne cycloaddition (RuAAC) is an emerging tool for organic and medicinal chemistry that provides access to 1,5-disubstituted 1,2,3-triazole motifs for applications in peptide engineering. Despite its growing popularity, investigation of this motif as a disulfide mimetic in backbone cyclic peptides has been limited, and structural consequences remain to be studied. Here, we establish synthetic strategies to install various triazole linkages in cyclic peptides using backbone cyclisation and RuAAC cross-linking reactions. These linkages were evaluated in four serine protease inhibitors based on the sunflower trypsin inhibitor-1 framework. NMR and X-ray crystallography revealed exceptional consensus of bridging distance and backbone conformations (RMSD <0.5 A) of the triazole linkages when compared to the parent disulfide molecules. The triazole-bridged peptides displayed superior half-lives in liver S9 stability assays compared to disulfide-bridged peptides. This work establishes a strong foundation for the application of 1,5-disubstituted 1,2,3-triazoles as disulfide mimetics. | ||
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| + | Application and Structural Analysis of Triazole-Bridged Disulfide Mimetics in Cyclic Peptides.,White A, de Veer S, Wu G, Harvey P, Yap K, King GJ, Swedberg JE, Wang CK, Law RHP, Durek T, Craik D Angew Chem Int Ed Engl. 2020 Apr 8. doi: 10.1002/anie.202003435. PMID:32270580<ref>PMID:32270580</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6u7r" style="background-color:#fffaf0;"></div> | ||
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| + | ==See Also== | ||
| + | *[[Trypsin inhibitor 3D structures|Trypsin inhibitor 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Helianthus annuus]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Craik DJ]] | [[Category: Craik DJ]] | ||
Revision as of 11:04, 14 June 2023
NMR solution structure of SFTI1 based KLK7 protease inhibitor
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