1l7d
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1l7d.gif|left|200px]] | [[Image:1l7d.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1l7d", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | | | + | --> |
| - | | | + | {{STRUCTURE_1l7d| PDB=1l7d | SCENE= }} |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''Crystal Structure of R. rubrum Transhydrogenase Domain I without Bound NAD(H)''' | '''Crystal Structure of R. rubrum Transhydrogenase Domain I without Bound NAD(H)''' | ||
| Line 23: | Line 20: | ||
==Reference== | ==Reference== | ||
Crystal structures of transhydrogenase domain I with and without bound NADH., Prasad GS, Wahlberg M, Sridhar V, Sundaresan V, Yamaguchi M, Hatefi Y, Stout CD, Biochemistry. 2002 Oct 22;41(42):12745-54. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12379117 12379117] | Crystal structures of transhydrogenase domain I with and without bound NADH., Prasad GS, Wahlberg M, Sridhar V, Sundaresan V, Yamaguchi M, Hatefi Y, Stout CD, Biochemistry. 2002 Oct 22;41(42):12745-54. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12379117 12379117] | ||
| - | [[Category: NAD(P)(+) transhydrogenase (B-specific)]] | ||
[[Category: Rhodospirillum rubrum]] | [[Category: Rhodospirillum rubrum]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 32: | Line 28: | ||
[[Category: Wahlberg, M.]] | [[Category: Wahlberg, M.]] | ||
[[Category: Yamaguchi, M.]] | [[Category: Yamaguchi, M.]] | ||
| - | [[Category: | + | [[Category: Transhydrogenase domain i]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:37:42 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 20:37, 2 May 2008
Crystal Structure of R. rubrum Transhydrogenase Domain I without Bound NAD(H)
Overview
Transhydrogenase (TH) is a dimeric integral membrane enzyme in mitochondria and prokaryotes that couples proton translocation across a membrane with hydride transfer between NAD(H) and NADP(H) in soluble domains. Crystal structures of the NAD(H) binding alpha1 subunit (domain I) of Rhodospirillum rubrum TH have been determined at 1.8 A resolution in the absence of dinucleotide and at 1.9 A resolution with NADH bound. Each structure contains two domain I dimers in the asymmetric unit (AB and CD); the dimers are intimately associated and related by noncrystallographic 2-fold axes. NADH binds to subunits A and D, consistent with the half-of-the-sites reactivity of the enzyme. The conformation of NADH in subunits A and D is very similar; the nicotinamide is in the anti conformation, the A-face is exposed to solvent, and both N7 and O7 participate in hydrogen bonds. Comparison of subunits A and D to six independent copies of the subunit without bound NADH reveals multiple conformations for residues and loops surrounding the NADH site, indicating flexibility for binding and release of the substrate (product). The NADH-bound structure is also compared to the structures of R. rubrum domain I with NAD bound (PDB code 1F8G) and with NAD bound in complex with domain III of TH (PDB code 1HZZ). The NADH- vs NAD-bound domain I structures reveal conformational differences in conserved residues in the NAD(H) binding site and in dinucleotide conformation that are correlated with the net charge, i.e., oxidation state, of the nicotinamides. The comparisons illustrate how nicotinamide oxidation state can affect the domain I conformation, which is relevant to the hydride transfer step of the overall reaction.
About this Structure
1L7D is a Single protein structure of sequence from Rhodospirillum rubrum. Full crystallographic information is available from OCA.
Reference
Crystal structures of transhydrogenase domain I with and without bound NADH., Prasad GS, Wahlberg M, Sridhar V, Sundaresan V, Yamaguchi M, Hatefi Y, Stout CD, Biochemistry. 2002 Oct 22;41(42):12745-54. PMID:12379117 Page seeded by OCA on Fri May 2 23:37:42 2008
