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| | ==Solution structure of Legionella pneumophila NttA== | | ==Solution structure of Legionella pneumophila NttA== |
| - | <StructureSection load='6xtt' size='340' side='right'caption='[[6xtt]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | + | <StructureSection load='6xtt' size='340' side='right'caption='[[6xtt]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6xtt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_33152 Atcc 33152]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XTT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XTT FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6xtt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Legionella_pneumophila Legionella pneumophila]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XTT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XTT FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">C3926_06540, C3927_06155, D7214_02725, DI056_01550, DI105_01550, ERS240541_00078, ERS253249_02720, NCTC12000_01505, NCTC12024_01284 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=446 ATCC 33152])</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xtt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xtt OCA], [https://pdbe.org/6xtt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xtt RCSB], [https://www.ebi.ac.uk/pdbsum/6xtt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xtt ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xtt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xtt OCA], [https://pdbe.org/6xtt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xtt RCSB], [https://www.ebi.ac.uk/pdbsum/6xtt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xtt ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q5ZVQ5_LEGPH Q5ZVQ5_LEGPH] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 33152]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Garnett, J A]] | |
| - | [[Category: Portlock, T J]] | |
| | [[Category: Legionella pneumophila]] | | [[Category: Legionella pneumophila]] |
| - | [[Category: Type ii secretion system]] | + | [[Category: Garnett JA]] |
| - | [[Category: Unknown function]] | + | [[Category: Portlock TJ]] |
| - | [[Category: Virulence factor]]
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| Structural highlights
Function
Q5ZVQ5_LEGPH
Publication Abstract from PubMed
Legionella pneumophila is a Gram-negative bacterium that is able to replicate within a broad range of aquatic protozoan hosts. L. pneumophila is also an opportunistic human pathogen that can infect macrophages and epithelia in the lung and lead to Legionnaires' disease. The type II secretion system is a key virulence factor of L. pneumophila and is used to promote bacterial growth at low temperatures, regulate biofilm formation, modulate host responses to infection, facilitate bacterial penetration of mucin gels and is necessary for intracellular growth during the initial stages of infection. The L. pneumophila type II secretion system exports at least 25 substrates out of the bacterium and several of these, including NttA to NttG, contain unique amino acid sequences that are generally not observed outside of the Legionella genus. NttA, NttC, and NttD are required for infection of several amoebal species but it is unclear what influence other novel substrates have within their host. In this study, we show that NttE is required for optimal infection of Acanthamoeba castellanii and Vermamoeba vermiformis amoeba and is essential for the typical colony morphology of L. pneumophila. In addition, we report the atomic structures of NttA, NttC, and NttE and through a combined biophysical and biochemical hypothesis driven approach we propose novel functions for these substrates during infection. This work lays the foundation for future studies into the mechanistic understanding of novel type II substrate functions and how these relate to L. pneumophila ecology and disease.
Structure, Dynamics and Cellular Insight Into Novel Substrates of the Legionella pneumophila Type II Secretion System.,Portlock TJ, Tyson JY, Dantu SC, Rehman S, White RC, McIntire IE, Sewell L, Richardson K, Shaw R, Pandini A, Cianciotto NP, Garnett JA Front Mol Biosci. 2020 Jun 11;7:112. doi: 10.3389/fmolb.2020.00112. eCollection, 2020. PMID:32656228[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Portlock TJ, Tyson JY, Dantu SC, Rehman S, White RC, McIntire IE, Sewell L, Richardson K, Shaw R, Pandini A, Cianciotto NP, Garnett JA. Structure, Dynamics and Cellular Insight Into Novel Substrates of the Legionella pneumophila Type II Secretion System. Front Mol Biosci. 2020 Jun 11;7:112. doi: 10.3389/fmolb.2020.00112. eCollection, 2020. PMID:32656228 doi:http://dx.doi.org/10.3389/fmolb.2020.00112
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