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7ckd
From Proteopedia
(Difference between revisions)
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<StructureSection load='7ckd' size='340' side='right'caption='[[7ckd]]' scene=''> | <StructureSection load='7ckd' size='340' side='right'caption='[[7ckd]]' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full | + | <table><tr><td colspan='2'>[[7ckd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Medicago_truncatula Medicago truncatula]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CKD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CKD FirstGlance]. <br> |
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ckd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ckd OCA], [https://pdbe.org/7ckd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ckd RCSB], [https://www.ebi.ac.uk/pdbsum/7ckd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ckd ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ckd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ckd OCA], [https://pdbe.org/7ckd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ckd RCSB], [https://www.ebi.ac.uk/pdbsum/7ckd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ckd ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A7KHA7_MEDTR A7KHA7_MEDTR] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | A model legume, Medicago truncatula, has over 600 nodule-specific cysteine-rich (NCR) peptides required for symbiosis with rhizobia. Among them, NCR169, an essential factor for establishing symbiosis, has four cysteine residues that are indispensable for its function. However, knowledge of NCR169 structure and mechanism of action is still lacking. In this study, we solved two NMR structures of NCR169 caused by different disulfide linkage patterns. We show that both structures have a consensus C-terminal beta-sheet attached to an extended N-terminal region with dissimilar features; one moves widely, whereas the other is relatively stapled. We further revealed that the disulfide bonds of NCR169 contribute to its structural stability and solubility. Regarding the function, one of the NCR169 oxidized forms could bind to negatively charged bacterial phospholipids. Furthermore, the positively charged lysine-rich region of NCR169 may be responsible for its antimicrobial activity against Escherichia coli and Sinorhizobium meliloti. This active region was disordered even in the phospholipid bound state, suggesting that the disordered conformation of this region is key to its function. Morphological observations suggested the mechanism of action of NCR169 on bacteria. The present study on NCR169 provides new insights into the structure and function of NCR peptides. | ||
| + | |||
| + | Structure and antimicrobial activity of NCR169, a nodule-specific cysteine-rich peptide of Medicago truncatula.,Isozumi N, Masubuchi Y, Imamura T, Mori M, Koga H, Ohki S Sci Rep. 2021 May 10;11(1):9923. doi: 10.1038/s41598-021-89485-w. PMID:33972675<ref>PMID:33972675</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7ckd" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Medicago truncatula]] | ||
[[Category: Isozumi N]] | [[Category: Isozumi N]] | ||
[[Category: Masubuchi Y]] | [[Category: Masubuchi Y]] | ||
[[Category: Ohki S]] | [[Category: Ohki S]] | ||
Revision as of 11:12, 14 June 2023
Solution structure of NCR169 oxidized form 1 from Medicago truncatula
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