7pre

From Proteopedia

(Difference between revisions)

Revision as of 11:19, 14 June 2023

Solution structure of the NRDI domain of Nab3

Structural highlights

7pre is a 1 chain structure with sequence from Saccharomyces cerevisiae. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NAB3_YEAST May be required for packaging pre-mRNAs into ribonucleoprotein structures amenable to efficient nuclear RNA processing. Binds to poly(A)+ RNA. Appears to act in the maintenance of CLN3 mRNA levels.^[1]

Publication Abstract from PubMed

Heterodimerization of RNA binding proteins Nrd1 and Nab3 is essential to communicate the RNA recognition in the nascent transcript with the Nrd1 recognition of the Ser5-phosphorylated Rbp1 C-terminal domain in RNA polymerase II. The structure of a Nrd1-Nab3 chimera reveals the basis of heterodimerization, filling a missing gap in knowledge of this system. The free form of the Nrd1 interaction domain of Nab3 (NRID) forms a multi-state three-helix bundle that is clamped in a single conformation upon complex formation with the Nab3 interaction domain of Nrd1 (NAID). The latter domain forms two long helices that wrap around NRID, resulting in an extensive protein-protein interface that would explain the highly favorable free energy of heterodimerization. Mutagenesis of some conserved hydrophobic residues involved in the heterodimerization leads to temperature-sensitive phenotypes, revealing the importance of this interaction in yeast cell fitness. The Nrd1-Nab3 structure resembles the previously reported Rna14/Rna15 heterodimer structure, which is part of the poly(A)-dependent termination pathway, suggesting that both machineries use similar structural solutions despite they share little sequence homology and are potentially evolutionary divergent.

Structural basis of Nrd1-Nab3 heterodimerization.,Chaves-Arquero B, Martinez-Lumbreras S, Camero S, Santiveri CM, Mirassou Y, Campos-Olivas R, Jimenez MA, Calvo O, Perez-Canadillas JM Life Sci Alliance. 2022 Jan 12;5(4). pii: 5/4/e202101252. doi:, 10.26508/lsa.202101252. Print 2022 Apr. PMID:35022249^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Sugimoto K, Matsumoto K, Kornberg RD, Reed SI, Wittenberg C. Dosage suppressors of the dominant G1 cyclin mutant CLN3-2: identification of a yeast gene encoding a putative RNA/ssDNA binding protein. Mol Gen Genet. 1995 Oct 25;248(6):712-8. PMID:7476874
↑ Chaves-Arquero B, Martinez-Lumbreras S, Camero S, Santiveri CM, Mirassou Y, Campos-Olivas R, Jimenez MA, Calvo O, Perez-Canadillas JM. Structural basis of Nrd1-Nab3 heterodimerization. Life Sci Alliance. 2022 Jan 12;5(4). pii: 5/4/e202101252. doi:, 10.26508/lsa.202101252. Print 2022 Apr. PMID:35022249 doi:http://dx.doi.org/10.26508/lsa.202101252

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/7pre"

Categories: Large Structures | Saccharomyces cerevisiae | Chaves-Arquero B | Martinez-Lumbreras S | Perez-Canadillas JM

@@ Line 1: / Line 1: @@
 ==Solution structure of the NRDI domain of Nab3==
-<StructureSection load='7pre' size='340' side='right'caption='[[7pre]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='7pre' size='340' side='right'caption='[[7pre]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[7pre]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PRE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PRE FirstGlance]. <br>
+<table><tr><td colspan='2'>[[7pre]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PRE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PRE FirstGlance]. <br>
 </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pre FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pre OCA], [https://pdbe.org/7pre PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pre RCSB], [https://www.ebi.ac.uk/pdbsum/7pre PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pre ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/NAB3_YEAST NAB3_YEAST] May be required for packaging pre-mRNAs into ribonucleoprotein structures amenable to efficient nuclear RNA processing. Binds to poly(A)+ RNA. Appears to act in the maintenance of CLN3 mRNA levels.<ref>PMID:7476874</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 20: / Line 22: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Chaves-Arquero, B]]
+[[Category: Saccharomyces cerevisiae]]
-[[Category: Martinez-Lumbreras, S]]
+[[Category: Chaves-Arquero B]]
-[[Category: Perez-Canadillas, J M]]
+[[Category: Martinez-Lumbreras S]]
-[[Category: Heterodimerization domain]]
+[[Category: Perez-Canadillas JM]]
-[[Category: Nab3]]
-[[Category: Nns pathway]]
-[[Category: Transcription]]
-[[Category: Transcription termination]]
-[[Category: Yeast]]

7pre

From Proteopedia

Revision as of 11:19, 14 June 2023

Solution structure of the NRDI domain of Nab3

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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