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| | ==Solution Structure of Sds3 Capped Tudor Domain== | | ==Solution Structure of Sds3 Capped Tudor Domain== |
| - | <StructureSection load='7sxi' size='340' side='right'caption='[[7sxi]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='7sxi' size='340' side='right'caption='[[7sxi]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[7sxi]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SXI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SXI FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7sxi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SXI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SXI FirstGlance]. <br> |
| | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sxi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sxi OCA], [https://pdbe.org/7sxi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sxi RCSB], [https://www.ebi.ac.uk/pdbsum/7sxi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sxi ProSAT]</span></td></tr> | | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sxi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sxi OCA], [https://pdbe.org/7sxi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sxi RCSB], [https://www.ebi.ac.uk/pdbsum/7sxi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sxi ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/SDS3_MOUSE SDS3_MOUSE]] Regulatory protein which represses transcription and augments histone deacetylase activity of HDAC1. May have a potential role in tumor suppressor pathways through regulation of apoptosis. May function in the assembly and/or enzymatic activity of the mSin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes (By similarity).
| + | [https://www.uniprot.org/uniprot/SDS3_MOUSE SDS3_MOUSE] Regulatory protein which represses transcription and augments histone deacetylase activity of HDAC1. May have a potential role in tumor suppressor pathways through regulation of apoptosis. May function in the assembly and/or enzymatic activity of the mSin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes (By similarity). |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Marcum, R D]] | + | [[Category: Mus musculus]] |
| - | [[Category: Radhakrishnan, I]] | + | [[Category: Marcum RD]] |
| - | [[Category: G-quadruplex binding]] | + | [[Category: Radhakrishnan I]] |
| - | [[Category: Gene regulation]]
| + | |
| - | [[Category: Nucleic acid binding]]
| + | |
| - | [[Category: Transcriptional corepressor]]
| + | |
| - | [[Category: Tudor domain]]
| + | |
| Structural highlights
Function
SDS3_MOUSE Regulatory protein which represses transcription and augments histone deacetylase activity of HDAC1. May have a potential role in tumor suppressor pathways through regulation of apoptosis. May function in the assembly and/or enzymatic activity of the mSin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes (By similarity).
Publication Abstract from PubMed
Chromatin-modifying complexes containing histone deacetylase (HDAC) activities play critical roles in the regulation of gene transcription in eukaryotes. These complexes are thought to lack intrinsic DNA-binding activity, but according to a well-established paradigm, they are recruited via protein-protein interactions by gene-specific transcription factors and post-translational histone modifications to their sites of action on the genome. The mammalian Sin3L/Rpd3L complex, comprising more than a dozen different polypeptides, is an ancient HDAC complex found in diverse eukaryotes. The subunits of this complex harbor conserved domains and motifs of unknown structure and function. Here we show that Sds3, a constitutively-associated subunit critical for the proper functioning of the Sin3L/Rpd3L complex, harbors a type of Tudor domain that we designate the capped Tudor domain (CTD). Unlike canonical Tudor domains that bind modified histones, the Sds3 CTD binds to nucleic acids that can form higher-order structures such as G-quadruplexes, and shares similarities with the knotted Tudor domain of the Esa1 histone acetyltransferase (HAT) that was previously shown to bind single-stranded RNA. Our findings expand the range of macromolecules capable of recruiting the Sin3L/Rpd3L complex and draw attention to potentially new biological roles for this HDAC complex.
A Capped Tudor Domain within a Core Subunit of the Sin3L/Rpd3L Histone Deacetylase Complex Binds to Nucleic Acid G-Quadruplexes.,Marcum RD, Hsieh J, Giljen M, Justice E, Daffern N, Zhang Y, Radhakrishnan I J Biol Chem. 2021 Dec 31:101558. doi: 10.1016/j.jbc.2021.101558. PMID:34979096[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Marcum RD, Hsieh J, Giljen M, Justice E, Daffern N, Zhang Y, Radhakrishnan I. A Capped Tudor Domain within a Core Subunit of the Sin3L/Rpd3L Histone Deacetylase Complex Binds to Nucleic Acid G-Quadruplexes. J Biol Chem. 2021 Dec 31:101558. doi: 10.1016/j.jbc.2021.101558. PMID:34979096 doi:http://dx.doi.org/10.1016/j.jbc.2021.101558
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