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| | <StructureSection load='5cht' size='340' side='right'caption='[[5cht]], [[Resolution|resolution]] 2.80Å' scene=''> | | <StructureSection load='5cht' size='340' side='right'caption='[[5cht]], [[Resolution|resolution]] 2.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5cht]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CHT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5CHT FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5cht]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CHT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CHT FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Usp18, Ubp43 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5cht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cht OCA], [https://pdbe.org/5cht PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5cht RCSB], [https://www.ebi.ac.uk/pdbsum/5cht PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5cht ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5cht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cht OCA], [http://pdbe.org/5cht PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5cht RCSB], [http://www.ebi.ac.uk/pdbsum/5cht PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5cht ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/UBP18_MOUSE UBP18_MOUSE]] Can efficiently cleave only ISG15 fusions including native ISG15 conjugates linked via isopeptide bonds. Necessary to maintain a critical cellular balance of ISG15-conjugated proteins in both healthy and stressed organisms (By similarity). | + | [https://www.uniprot.org/uniprot/UBP18_MOUSE UBP18_MOUSE] Can efficiently cleave only ISG15 fusions including native ISG15 conjugates linked via isopeptide bonds. Necessary to maintain a critical cellular balance of ISG15-conjugated proteins in both healthy and stressed organisms (By similarity). |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Mus musculus]] |
| - | [[Category: Basters, A]] | + | [[Category: Basters A]] |
| - | [[Category: Fritz, G]] | + | [[Category: Fritz G]] |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Interferon]]
| + | |
| - | [[Category: Isg15]]
| + | |
| - | [[Category: Ubiquitin-specific protease]]
| + | |
| Structural highlights
Function
UBP18_MOUSE Can efficiently cleave only ISG15 fusions including native ISG15 conjugates linked via isopeptide bonds. Necessary to maintain a critical cellular balance of ISG15-conjugated proteins in both healthy and stressed organisms (By similarity).
Publication Abstract from PubMed
Protein modification by ubiquitin and ubiquitin-like modifiers (Ubls) is counteracted by ubiquitin proteases and Ubl proteases, collectively termed DUBs. In contrast to other proteases of the ubiquitin-specific protease (USP) family, USP18 shows no reactivity toward ubiquitin but specifically deconjugates the interferon-induced Ubl ISG15. To identify the molecular determinants of this specificity, we solved the crystal structures of mouse USP18 alone and in complex with mouse ISG15. USP18 was crystallized in an open and a closed conformation, thus revealing high flexibility of the enzyme. Structural data, biochemical and mutational analysis showed that only the C-terminal ubiquitin-like domain of ISG15 is recognized and essential for USP18 activity. A critical hydrophobic patch in USP18 interacts with a hydrophobic region unique to ISG15, thus providing evidence that USP18's ISG15 specificity is mediated by a small interaction interface. Our results may provide a structural basis for the development of new drugs modulating ISG15 linkage.
Structural basis of the specificity of USP18 toward ISG15.,Basters A, Geurink PP, Rocker A, Witting KF, Tadayon R, Hess S, Semrau MS, Storici P, Ovaa H, Knobeloch KP, Fritz G Nat Struct Mol Biol. 2017 Mar;24(3):270-278. doi: 10.1038/nsmb.3371. Epub 2017, Feb 6. PMID:28165509[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Basters A, Geurink PP, Rocker A, Witting KF, Tadayon R, Hess S, Semrau MS, Storici P, Ovaa H, Knobeloch KP, Fritz G. Structural basis of the specificity of USP18 toward ISG15. Nat Struct Mol Biol. 2017 Mar;24(3):270-278. doi: 10.1038/nsmb.3371. Epub 2017, Feb 6. PMID:28165509 doi:http://dx.doi.org/10.1038/nsmb.3371
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