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| | <StructureSection load='5cl2' size='340' side='right'caption='[[5cl2]], [[Resolution|resolution]] 2.30Å' scene=''> | | <StructureSection load='5cl2' size='340' side='right'caption='[[5cl2]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5cl2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacsu Bacsu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CL2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5CL2 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5cl2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CL2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CL2 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">spo0M, ygaI, BSU08760 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5cl2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cl2 OCA], [https://pdbe.org/5cl2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5cl2 RCSB], [https://www.ebi.ac.uk/pdbsum/5cl2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5cl2 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5cl2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cl2 OCA], [http://pdbe.org/5cl2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5cl2 RCSB], [http://www.ebi.ac.uk/pdbsum/5cl2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5cl2 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/SP0M_BACSU SP0M_BACSU]] Controls the expression of spo0A and is required to pass the morphological stage 0 of sporulation.<ref>PMID:9795118</ref> | + | [https://www.uniprot.org/uniprot/SP0M_BACSU SP0M_BACSU] Controls the expression of spo0A and is required to pass the morphological stage 0 of sporulation.<ref>PMID:9795118</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacsu]] | + | [[Category: Bacillus subtilis subsp. subtilis str. 168]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Mikami, B]] | + | [[Category: Mikami B]] |
| - | [[Category: Mizutani, K]] | + | [[Category: Mizutani K]] |
| - | [[Category: Sonoda, Y]] | + | [[Category: Sonoda Y]] |
| - | [[Category: Protein binding]]
| + | |
| - | [[Category: Spo0m]]
| + | |
| - | [[Category: Sporulation]]
| + | |
| Structural highlights
Function
SP0M_BACSU Controls the expression of spo0A and is required to pass the morphological stage 0 of sporulation.[1]
Publication Abstract from PubMed
Spo0M is a sporulation-control protein that is thought to play an essential role in the early stage of endospore formation. While little is known about the functions of Spo0M, a recent phylogenetic study suggests that, based on its amino-acid sequence, Spo0M might belong to the arrestin clan. The crystal structure of the Spo0M protein was determined at a resolution of 2.3 A. Ten amino acids at the end of the N-terminus were removed to improve the thermal stability of the purified Spo0M protein and the crystal structure of Spo0M was determined by SAD. Spo0M has a well conserved N-terminal domain with an arrestin-like fold, which consists of a beta-strand sandwich structure. Surprisingly, the C-terminal domain of Spo0M, which has no structural homology to arrestin-clan proteins, bears significant structural similarity to the FP domain of the human PI31 protein. In addition, Spo0M harbours a potential polar-core structure connecting the N- and C-terminal domains with several salt bridges, as seen in the crystal structures of arrestin and VPS26. The structure reported here constitutes the first structural information on a bacterial protein that shares significant structural homology to members of the arrestin clan and the FP domain.
Structure of Spo0M, a sporulation-control protein from Bacillus subtilis.,Sonoda Y, Mizutani K, Mikami B Acta Crystallogr F Struct Biol Commun. 2015 Dec 1;71(Pt 12):1488-97. doi:, 10.1107/S2053230X15020919. Epub 2015 Nov 18. PMID:26625291[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Han WD, Kawamoto S, Hosoya Y, Fujita M, Sadaie Y, Suzuki K, Ohashi Y, Kawamura F, Ochi K. A novel sporulation-control gene (spo0M) of Bacillus subtilis with a sigmaH-regulated promoter. Gene. 1998 Sep 14;217(1-2):31-40. PMID:9795118
- ↑ Sonoda Y, Mizutani K, Mikami B. Structure of Spo0M, a sporulation-control protein from Bacillus subtilis. Acta Crystallogr F Struct Biol Commun. 2015 Dec 1;71(Pt 12):1488-97. doi:, 10.1107/S2053230X15020919. Epub 2015 Nov 18. PMID:26625291 doi:http://dx.doi.org/10.1107/S2053230X15020919
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