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| | <StructureSection load='5cm8' size='340' side='right'caption='[[5cm8]], [[Resolution|resolution]] 2.60Å' scene=''> | | <StructureSection load='5cm8' size='340' side='right'caption='[[5cm8]], [[Resolution|resolution]] 2.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5cm8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome] and [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CM8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5CM8 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5cm8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CM8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CM8 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5cm9|5cm9]]</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5cm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cm8 OCA], [https://pdbe.org/5cm8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5cm8 RCSB], [https://www.ebi.ac.uk/pdbsum/5cm8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5cm8 ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Rgl2, Rab2l, Rlf ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice]), Rala, CG2849 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5cm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cm8 OCA], [http://pdbe.org/5cm8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5cm8 RCSB], [http://www.ebi.ac.uk/pdbsum/5cm8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5cm8 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/RGL2_MOUSE RGL2_MOUSE]] Probable guanine nucleotide exchange factor. Putative effector of Ras and/or Rap. Associates with the GTP-bound form of Rap 1A and H-Ras in vitro. | + | [https://www.uniprot.org/uniprot/RGL2_MOUSE RGL2_MOUSE] Probable guanine nucleotide exchange factor. Putative effector of Ras and/or Rap. Associates with the GTP-bound form of Rap 1A and H-Ras in vitro. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Drome]] | + | [[Category: Drosophila melanogaster]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Mus musculus]] |
| - | [[Category: Popovic, M]] | + | [[Category: Popovic M]] |
| - | [[Category: Rehmann, H]] | + | [[Category: Rehmann H]] |
| - | [[Category: Schouten, A]] | + | [[Category: Schouten A]] |
| - | [[Category: Complex g-protein exchange factor]]
| + | |
| - | [[Category: Signaling protein]]
| + | |
| Structural highlights
Function
RGL2_MOUSE Probable guanine nucleotide exchange factor. Putative effector of Ras and/or Rap. Associates with the GTP-bound form of Rap 1A and H-Ras in vitro.
Publication Abstract from PubMed
CDC25 homology domain (CDC25-HD) containing Guanine Nucleotide Exchange Factors (GEFs) initiate signalling by small G-proteins of the Ras-family. Each GEF acts on a small subset of the G-proteins only, thus providing signalling selectivity. Rlf is a GEF with selectivity for the G-proteins RalA and RalB. Here the crystal structure of Rlf in complex with Ral is determined. The Rlf.Ral complex crystallised into two different crystal forms, which represent different steps of the exchange reaction. Thereby general insight in the CDC25-HD catalysed nucleotide exchange is obtained. In addition, the basis for the selectivity of the interaction is investigated. The exchange activity is monitored by the use of recombinant proteins. Selectivity determinants in the binding interface are identified and confirmed by a mutational study.
The structure of the Guanine Nucleotide Exchange Factor Rlf in complex with the small G-protein Ral identifies conformational intermediates of the exchange reaction and the basis for the selectivity.,Popovic M, Schouten A, Rensen-de Leeuw M, Rehmann H J Struct Biol. 2015 Dec 11. pii: S1047-8477(15)30112-X. doi:, 10.1016/j.jsb.2015.12.006. PMID:26687416[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Popovic M, Schouten A, Rensen-de Leeuw M, Rehmann H. The structure of the Guanine Nucleotide Exchange Factor Rlf in complex with the small G-protein Ral identifies conformational intermediates of the exchange reaction and the basis for the selectivity. J Struct Biol. 2015 Dec 11. pii: S1047-8477(15)30112-X. doi:, 10.1016/j.jsb.2015.12.006. PMID:26687416 doi:http://dx.doi.org/10.1016/j.jsb.2015.12.006
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