1l8l

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:1l8l.jpg|left|200px]]
[[Image:1l8l.jpg|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 1l8l |SIZE=350|CAPTION= <scene name='initialview01'>1l8l</scene>, resolution 2.51&Aring;
+
The line below this paragraph, containing "STRUCTURE_1l8l", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=APO:D-2-AMINO-3-PHOSPHONO-PROPIONIC+ACID'>APO</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoserine_phosphatase Phosphoserine phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.3 3.1.3.3] </span>
+
or leave the SCENE parameter empty for the default display.
-
|GENE=
+
-->
-
|DOMAIN=
+
{{STRUCTURE_1l8l| PDB=1l8l | SCENE= }}
-
|RELATEDENTRY=[[1l8o|1L8O]]
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1l8l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l8l OCA], [http://www.ebi.ac.uk/pdbsum/1l8l PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1l8l RCSB]</span>
+
-
}}
+
'''Molecular basis for the local confomational rearrangement of human phosphoserine phosphatase'''
'''Molecular basis for the local confomational rearrangement of human phosphoserine phosphatase'''
Line 36: Line 33:
[[Category: Park, S Y.]]
[[Category: Park, S Y.]]
[[Category: Ro, S.]]
[[Category: Ro, S.]]
-
[[Category: conformational rearrangement]]
+
[[Category: Conformational rearrangement]]
-
[[Category: phosphatase]]
+
[[Category: Phosphatase]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:40:07 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:59:34 2008''
+

Revision as of 20:40, 2 May 2008

Image:1l8l.jpg

Template:STRUCTURE 1l8l

Molecular basis for the local confomational rearrangement of human phosphoserine phosphatase


Overview

Human phosphoserine phosphatase (HPSP) regulates the levels of glycine and d-serine, the putative co-agonists for the glycine site of the NMDA receptor in the brain. Here, we describe the first crystal structures of the HPSP in complexes with the competitive inhibitor 2-amino-3-phosphonopropionic acid (AP3) at 2.5 A, and the phosphate ion (Pi) and the product uncompetitive inhibitor l-serine (HPSP.l-Ser.Pi) at 2.8 A. The complex structures reveal that the open-closed environmental change of the active site, generated by local rearrangement of the alpha-helical bundle domain, is important to substrate recognition and hydrolysis. The maximal extent of this structural rearrangement is shown to be about 13 A at the L4 loop and about 25 degrees at the helix alpha3. Both the structural change and mutagenesis data suggest that Arg-65 and Glu-29 play an important role in the binding of the substrate. Interestingly, the AP3 binding mode turns out to be significantly different from that of the natural substrate, phospho-l-serine, and the HPSP.l-Ser.Pi structure provides a structural basis for the feedback control mechanism of serine. These analyses allow us to provide a clear model for the mechanism of HPSP and a framework for structure-based drug development.

About this Structure

1L8L is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Molecular basis for the local conformational rearrangement of human phosphoserine phosphatase., Kim HY, Heo YS, Kim JH, Park MH, Moon J, Kim E, Kwon D, Yoon J, Shin D, Jeong EJ, Park SY, Lee TG, Jeon YH, Ro S, Cho JM, Hwang KY, J Biol Chem. 2002 Nov 29;277(48):46651-8. Epub 2002 Sep 3. PMID:12213811 Page seeded by OCA on Fri May 2 23:40:07 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1l8l"
Personal tools