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| | <StructureSection load='5cpc' size='340' side='right'caption='[[5cpc]], [[Resolution|resolution]] 2.15Å' scene=''> | | <StructureSection load='5cpc' size='340' side='right'caption='[[5cpc]], [[Resolution|resolution]] 2.15Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5cpc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Salty Salty]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CPC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5CPC FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5cpc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium_str._LT2 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CPC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CPC FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5cq9|5cq9]]</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5cpc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cpc OCA], [https://pdbe.org/5cpc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5cpc RCSB], [https://www.ebi.ac.uk/pdbsum/5cpc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5cpc ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">sopD, STM2945 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=99287 SALTY])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5cpc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cpc OCA], [http://pdbe.org/5cpc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5cpc RCSB], [http://www.ebi.ac.uk/pdbsum/5cpc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5cpc ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/SOPD_SALTY SOPD_SALTY]] Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. Contributes to replication in macrophages. Plays a role, cooperatively with SopB, in membrane fission and macropinosome formation during invasion.<ref>PMID:15554961</ref> <ref>PMID:17696999</ref> | + | [https://www.uniprot.org/uniprot/SOPD_SALTY SOPD_SALTY] Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. Contributes to replication in macrophages. Plays a role, cooperatively with SopB, in membrane fission and macropinosome formation during invasion.<ref>PMID:15554961</ref> <ref>PMID:17696999</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Salty]] | + | [[Category: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]] |
| - | [[Category: Structural genomic]]
| + | [[Category: Cygler M]] |
| - | [[Category: Cygler, M]] | + | [[Category: Shi R]] |
| - | [[Category: Shi, R]] | + | |
| - | [[Category: Bsgi]]
| + | |
| - | [[Category: Cell invasion]]
| + | |
| - | [[Category: T3ss effector protein]]
| + | |
| Structural highlights
Function
SOPD_SALTY Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. Contributes to replication in macrophages. Plays a role, cooperatively with SopB, in membrane fission and macropinosome formation during invasion.[1] [2]
Publication Abstract from PubMed
Intracellular bacterial pathogens of a diverse nature share the ability to evade host immunity by impairing trafficking of endocytic cargo to lysosomes for degradation, a process that is poorly understood. Here, we show that the Salmonella enterica type 3 secreted effector SopD2 mediates this process by binding the host regulatory GTPase Rab7 and inhibiting its nucleotide exchange. Consequently, this limits Rab7 interaction with its dynein- and kinesin-binding effectors RILP and FYCO1 and thereby disrupts host-driven regulation of microtubule motors. Our study identifies a bacterial effector capable of directly binding and thereby modulating Rab7 activity and a mechanism of endocytic trafficking disruption that may provide insight into the pathogenesis of other bacteria. Additionally, we provide a powerful tool for the study of Rab7 function, and a potential therapeutic target.
Salmonella Disrupts Host Endocytic Trafficking by SopD2-Mediated Inhibition of Rab7.,D'Costa VM, Braun V, Landekic M, Shi R, Proteau A, McDonald L, Cygler M, Grinstein S, Brumell JH Cell Rep. 2015 Sep 1;12(9):1508-18. doi: 10.1016/j.celrep.2015.07.063. Epub 2015 , Aug 20. PMID:26299973[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Jiang X, Rossanese OW, Brown NF, Kujat-Choy S, Galan JE, Finlay BB, Brumell JH. The related effector proteins SopD and SopD2 from Salmonella enterica serovar Typhimurium contribute to virulence during systemic infection of mice. Mol Microbiol. 2004 Dec;54(5):1186-98. PMID:15554961 doi:http://dx.doi.org/10.1111/j.1365-2958.2004.04344.x
- ↑ Bakowski MA, Cirulis JT, Brown NF, Finlay BB, Brumell JH. SopD acts cooperatively with SopB during Salmonella enterica serovar Typhimurium invasion. Cell Microbiol. 2007 Dec;9(12):2839-55. Epub 2007 Aug 13. PMID:17696999 doi:http://dx.doi.org/10.1111/j.1462-5822.2007.01000.x
- ↑ D'Costa VM, Braun V, Landekic M, Shi R, Proteau A, McDonald L, Cygler M, Grinstein S, Brumell JH. Salmonella Disrupts Host Endocytic Trafficking by SopD2-Mediated Inhibition of Rab7. Cell Rep. 2015 Sep 1;12(9):1508-18. doi: 10.1016/j.celrep.2015.07.063. Epub 2015 , Aug 20. PMID:26299973 doi:http://dx.doi.org/10.1016/j.celrep.2015.07.063
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