1l9m
From Proteopedia
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'''Three-dimensional structure of the human transglutaminase 3 enzyme: binding of calcium ions change structure for activation''' | '''Three-dimensional structure of the human transglutaminase 3 enzyme: binding of calcium ions change structure for activation''' | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Ahvazi, B.]] | [[Category: Ahvazi, B.]] | ||
| - | [[Category: | + | [[Category: Activation]] |
| - | [[Category: | + | [[Category: Calcium binding]] |
| - | [[Category: | + | [[Category: Transglutaminase]] |
| - | [[Category: | + | [[Category: X-ray structure]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:42:05 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 20:42, 2 May 2008
Three-dimensional structure of the human transglutaminase 3 enzyme: binding of calcium ions change structure for activation
Overview
Transglutaminase (TGase) enzymes catalyze the formation of covalent cross-links between protein-bound glutamines and lysines in a calcium-dependent manner, but the role of Ca(2+) ions remains unclear. The TGase 3 isoform is widely expressed and is important for epithelial barrier formation. It is a zymogen, requiring proteolysis for activity. We have solved the three-dimensional structures of the zymogen and the activated forms at 2.2 and 2.1 A resolution, respectively, and examined the role of Ca(2+) ions. The zymogen binds one ion tightly that cannot be exchanged. Upon proteolysis, the enzyme exothermally acquires two more Ca(2+) ions that activate the enzyme, are exchangeable and are functionally replaceable by other lanthanide trivalent cations. Binding of a Ca(2+) ion at one of these sites opens a channel which exposes the key Trp236 and Trp327 residues that control substrate access to the active site. Together, these biochemical and structural data reveal for the first time in a TGase enzyme that Ca(2+) ions induce structural changes which at least in part dictate activity and, moreover, may confer substrate specificity.
About this Structure
1L9M is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Three-dimensional structure of the human transglutaminase 3 enzyme: binding of calcium ions changes structure for activation., Ahvazi B, Kim HC, Kee SH, Nemes Z, Steinert PM, EMBO J. 2002 May 1;21(9):2055-67. PMID:11980702 Page seeded by OCA on Fri May 2 23:42:05 2008
