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| | <StructureSection load='5cvy' size='340' side='right'caption='[[5cvy]], [[Resolution|resolution]] 2.00Å' scene=''> | | <StructureSection load='5cvy' size='340' side='right'caption='[[5cvy]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5cvy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacp2 Bacp2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CVY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5CVY FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5cvy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_pumilus_SAFR-032 Bacillus pumilus SAFR-032]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CVY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CVY FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CBI:CELLOBIOSE'>CBI</scene>, <scene name='pdbligand=CE6:CELLOHEXAOSE'>CE6</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PRD_900005:beta-cellobiose'>PRD_900005</scene>, <scene name='pdbligand=PRD_900020:beta-cellohexaose'>PRD_900020</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BPUM_1559 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=315750 BACP2])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5cvy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cvy OCA], [https://pdbe.org/5cvy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5cvy RCSB], [https://www.ebi.ac.uk/pdbsum/5cvy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5cvy ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5cvy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cvy OCA], [http://pdbe.org/5cvy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5cvy RCSB], [http://www.ebi.ac.uk/pdbsum/5cvy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5cvy ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A8FDC4_BACP2 A8FDC4_BACP2] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacp2]] | + | [[Category: Bacillus pumilus SAFR-032]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Alahuhta, P M]] | + | [[Category: Alahuhta PM]] |
| - | [[Category: Lunin, V V]] | + | [[Category: Lunin VV]] |
| - | [[Category: Cellulase]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| Structural highlights
5cvy is a 1 chain structure with sequence from Bacillus pumilus SAFR-032. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Ligands: | , , , , , , , |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
A8FDC4_BACP2
Publication Abstract from PubMed
Glycoside hydrolase (GH) family 48 is an understudied and increasingly important exoglucanase family found in the majority of bacterial cellulase systems. Moreover, many thermophilic enzyme systems contain GH48 enzymes. Deletion of GH48 enzymes in these microorganisms results in drastic reduction in biomass deconstruction. Surprisingly, given their importance for these microorganisms, GH48s have intrinsically low cellulolytic activity but even in low ratios synergize greatly with GH9 endoglucanases. In this study, we explore the structural and enzymatic diversity of these enzymes across a wide range of temperature optima. We have crystallized one new GH48 module from Bacillus pumilus in a complex with cellobiose and cellohexaose (BpumGH48). We compare this structure to other known GH48 enzymes in an attempt to understand GH48 structure/function relationships and draw general rules correlating amino acid sequences and secondary structures to thermostability in this GH family.
Natural diversity of glycoside hydrolase family 48 exoglucanases: insights from structure.,Brunecky R, Alahuhta M, Sammond DW, Xu Q, Chen M, Wilson DB, Brady JW, Himmel ME, Bomble YJ, Lunin VV Biotechnol Biofuels. 2017 Nov 30;10:274. doi: 10.1186/s13068-017-0951-5., eCollection 2017. PMID:29213319[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Brunecky R, Alahuhta M, Sammond DW, Xu Q, Chen M, Wilson DB, Brady JW, Himmel ME, Bomble YJ, Lunin VV. Natural diversity of glycoside hydrolase family 48 exoglucanases: insights from structure. Biotechnol Biofuels. 2017 Nov 30;10:274. doi: 10.1186/s13068-017-0951-5., eCollection 2017. PMID:29213319 doi:http://dx.doi.org/10.1186/s13068-017-0951-5
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