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5det

From Proteopedia

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Revision as of 10:32, 21 June 2023

X-ray structure of human RBPMS in complex with the RNA

Structural highlights

5det is a 4 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RBPMS_HUMAN Acts as a coactivator of transcriptional activity. Required to increase TGFB1/Smad-mediated transactivation. Acts through SMAD2, SMAD3 and SMAD4 to increase transcriptional activity. Increases phosphorylation of SMAD2 and SMAD3 on their C-terminal SSXS motif, possibly through recruitment of TGFBR1. Promotes the nuclear accumulation of SMAD2, SMAD3 and SMAD4 proteins. Binds to poly(A) RNA.^[1]

Publication Abstract from PubMed

RNA-binding protein with multiple splicing (designated RBPMS) is a higher vertebrate mRNA-binding protein containing a single RNA recognition motif (RRM). RBPMS has been shown to be involved in mRNA transport, localization and stability, with key roles in axon guidance, smooth muscle plasticity, as well as regulation of cancer cell proliferation and migration. We report on structure-function studies of the RRM domain of RBPMS bound to a CAC-containing single-stranded RNA. These results provide insights into potential topologies of complexes formed by the RBPMS RRM domain and the tandem CAC repeat binding sites as detected by photoactivatable-ribonucleoside-enhanced crosslinking and immunoprecipitation. These studies establish that the RRM domain of RBPMS forms a symmetrical dimer in the free state, with each monomer binding sequence-specifically to all three nucleotides of a CAC segment in the RNA bound state. Structure-guided mutations within the dimerization and RNA-binding interfaces of RBPMS RRM on RNA complex formation resulted in both disruption of dimerization and a decrease in RNA-binding affinity as observed by size exclusion chromatography and isothermal titration calorimetry. As anticipated from biochemical binding studies, over-expression of dimerization or RNA-binding mutants of Flag-HA-tagged RBPMS were no longer able to track with stress granules in HEK293 cells, thereby documenting the deleterious effects of such mutations in vivo.

Structural basis underlying CAC RNA recognition by the RRM domain of dimeric RNA-binding protein RBPMS.,Teplova M, Farazi TA, Tuschl T, Patel DJ Q Rev Biophys. 2015 Sep 8:1-11. PMID:26347403^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Sun Y, Ding L, Zhang H, Han J, Yang X, Yan J, Zhu Y, Li J, Song H, Ye Q. Potentiation of Smad-mediated transcriptional activation by the RNA-binding protein RBPMS. Nucleic Acids Res. 2006;34(21):6314-26. Epub 2006 Nov 11. PMID:17099224 doi:http://dx.doi.org/10.1093/nar/gkl914
↑ Teplova M, Farazi TA, Tuschl T, Patel DJ. Structural basis underlying CAC RNA recognition by the RRM domain of dimeric RNA-binding protein RBPMS. Q Rev Biophys. 2015 Sep 8:1-11. PMID:26347403 doi:http://dx.doi.org/10.1017/S0033583515000207

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5det"

@@ Line 3: / Line 3: @@
 <StructureSection load='5det' size='340' side='right'caption='[[5det]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5det]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DET OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5DET FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5det]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DET OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5DET FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5cyj|5cyj]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5det FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5det OCA], [https://pdbe.org/5det PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5det RCSB], [https://www.ebi.ac.uk/pdbsum/5det PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5det ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5det FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5det OCA], [http://pdbe.org/5det PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5det RCSB], [http://www.ebi.ac.uk/pdbsum/5det PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5det ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/RBPMS_HUMAN RBPMS_HUMAN]] Acts as a coactivator of transcriptional activity. Required to increase TGFB1/Smad-mediated transactivation. Acts through SMAD2, SMAD3 and SMAD4 to increase transcriptional activity. Increases phosphorylation of SMAD2 and SMAD3 on their C-terminal SSXS motif, possibly through recruitment of TGFBR1. Promotes the nuclear accumulation of SMAD2, SMAD3 and SMAD4 proteins. Binds to poly(A) RNA.<ref>PMID:17099224</ref>
+[https://www.uniprot.org/uniprot/RBPMS_HUMAN RBPMS_HUMAN] Acts as a coactivator of transcriptional activity. Required to increase TGFB1/Smad-mediated transactivation. Acts through SMAD2, SMAD3 and SMAD4 to increase transcriptional activity. Increases phosphorylation of SMAD2 and SMAD3 on their C-terminal SSXS motif, possibly through recruitment of TGFBR1. Promotes the nuclear accumulation of SMAD2, SMAD3 and SMAD4 proteins. Binds to poly(A) RNA.<ref>PMID:17099224</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 22: @@
 __TOC__
 </StructureSection>
+[[Category: Homo sapiens]]
 [[Category: Large Structures]]
-[[Category: Farazi, T A]]
+[[Category: Farazi TA]]
-[[Category: Patel, D J]]
+[[Category: Patel DJ]]
-[[Category: Teplova, M]]
+[[Category: Teplova M]]
-[[Category: Tuschl, T]]
+[[Category: Tuschl T]]
-[[Category: Rna binding protein]]
-[[Category: Rna binding protein-rna complex]]
-[[Category: Rrm domain]]

5det

From Proteopedia

Revision as of 10:32, 21 June 2023

X-ray structure of human RBPMS in complex with the RNA

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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