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| | <StructureSection load='5djn' size='340' side='right'caption='[[5djn]], [[Resolution|resolution]] 2.82Å' scene=''> | | <StructureSection load='5djn' size='340' side='right'caption='[[5djn]], [[Resolution|resolution]] 2.82Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5djn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DJN OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5DJN FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5djn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DJN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5DJN FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Kif13a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.82Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5djn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5djn OCA], [http://pdbe.org/5djn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5djn RCSB], [http://www.ebi.ac.uk/pdbsum/5djn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5djn ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5djn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5djn OCA], [https://pdbe.org/5djn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5djn RCSB], [https://www.ebi.ac.uk/pdbsum/5djn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5djn ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/F8VQ75_MOUSE F8VQ75_MOUSE] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Mus musculus]] |
| - | [[Category: Feng, W]] | + | [[Category: Feng W]] |
| - | [[Category: Ren, J Q]] | + | [[Category: Ren JQ]] |
| - | [[Category: Coil-coil]]
| + | |
| - | [[Category: Transport protein]]
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| Structural highlights
Function
F8VQ75_MOUSE
Publication Abstract from PubMed
Processive kinesin motors often contain a coiled-coil neck that controls the directionality and processivity. However, the neck coil (NC) of kinesin-3 is too short to form a stable coiled-coil dimer. Here, we found that the CC1-FHA tandem (that is connected to NC by P390) of kinesin-3 KIF13A assembles as an extended dimer. With the removal of P390, the NC-CC1 tandem of KIF13A unexpectedly forms a continuous coiled-coil dimer that can be well aligned into the CC1-FHA dimer. The reverse introduction of P390 breaks the NC-CC1 coiled-coil dimer but provides the intrinsic flexibility to couple NC with the CC1-FHA tandem. Mutations of either NC, CC1 or the FHA domain all significantly impaired the motor activity. Thus, the three elements within the NC-CC1-FHA tandem of KIF13A are structurally interrelated to form a stable dimer for activating the motor. This work also provides the first direct structural evidence to support the formation of a coiled-coil neck by the short characteristic neck domain of kinesin-3.
Structural correlation of the neck coil with the CC1-FHA tandem for active kinesin-3 KIF13A.,Ren J, Huo L, Wang W, Zhang Y, Li W, Lou J, Xu T, Feng W J Biol Chem. 2015 Dec 17. pii: jbc.M115.689091. PMID:26680000[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Ren J, Huo L, Wang W, Zhang Y, Li W, Lou J, Xu T, Feng W. Structural correlation of the neck coil with the CC1-FHA tandem for active kinesin-3 KIF13A. J Biol Chem. 2015 Dec 17. pii: jbc.M115.689091. PMID:26680000 doi:http://dx.doi.org/10.1074/jbc.M115.689091
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