1ldt

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[[Image:1ldt.gif|left|200px]]
[[Image:1ldt.gif|left|200px]]
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{{Structure
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|PDB= 1ldt |SIZE=350|CAPTION= <scene name='initialview01'>1ldt</scene>, resolution 1.9&Aring;
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The line below this paragraph, containing "STRUCTURE_1ldt", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span>
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{{STRUCTURE_1ldt| PDB=1ldt | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ldt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ldt OCA], [http://www.ebi.ac.uk/pdbsum/1ldt PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ldt RCSB]</span>
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'''COMPLEX OF LEECH-DERIVED TRYPTASE INHIBITOR WITH PORCINE TRYPSIN'''
'''COMPLEX OF LEECH-DERIVED TRYPTASE INHIBITOR WITH PORCINE TRYPSIN'''
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[[Category: Trypsin]]
[[Category: Trypsin]]
[[Category: Stubbs, M T.]]
[[Category: Stubbs, M T.]]
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[[Category: complex (hydrolase/inhibitor)]]
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[[Category: Hydrolase]]
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[[Category: hydrolase]]
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[[Category: Inflammation]]
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[[Category: inflammation]]
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[[Category: Inhibitor]]
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[[Category: inhibitor]]
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[[Category: Tryptase]]
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[[Category: tryptase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:49:40 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:01:36 2008''
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Revision as of 20:49, 2 May 2008

Template:STRUCTURE 1ldt

COMPLEX OF LEECH-DERIVED TRYPTASE INHIBITOR WITH PORCINE TRYPSIN


Overview

The x-ray crystal structure of recombinant leech-derived tryptase inhibitor (rLDTI) has been solved to a resolution of 1.9 A in complex with porcine trypsin. The nonclassical Kazal-type inhibitor exhibits the same overall architecture as that observed in solution and in rhodniin. The complex reveals structural aspects of the mast cell proteinase tryptase. The conformation of the binding region of rLDTI suggests that tryptase has a restricted active site cleft. The basic amino terminus of rLDTI, apparently flexible from previous NMR measurements, approaches the 148-loop of trypsin. This loop has an acidic equivalent in tryptase, suggesting that the basic amino terminus could make favorable electrostatic interactions with the tryptase molecule. A series of rLDTI variants constructed to probe this hypothesis confirmed that the amino-terminal Lys-Lys sequence plays a role in inhibition of human lung tryptase but not of trypsin or chymotrypsin. The location of such an acidic surface patch is in accordance with the known low molecular weight inhibitors of tryptase.

About this Structure

1LDT is a Protein complex structure of sequences from Hirudo medicinalis and Sus scrofa. Full crystallographic information is available from OCA.

Reference

The three-dimensional structure of recombinant leech-derived tryptase inhibitor in complex with trypsin. Implications for the structure of human mast cell tryptase and its inhibition., Stubbs MT, Morenweiser R, Sturzebecher J, Bauer M, Bode W, Huber R, Piechottka GP, Matschiner G, Sommerhoff CP, Fritz H, Auerswald EA, J Biol Chem. 1997 Aug 8;272(32):19931-7. PMID:9242660 Page seeded by OCA on Fri May 2 23:49:40 2008

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