1le8

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:1le8.jpg|left|200px]]
[[Image:1le8.jpg|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 1le8 |SIZE=350|CAPTION= <scene name='initialview01'>1le8</scene>, resolution 2.30&Aring;
+
The line below this paragraph, containing "STRUCTURE_1le8", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY=
+
or leave the SCENE parameter empty for the default display.
-
|GENE= MATalpha2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])
+
-->
-
|DOMAIN=
+
{{STRUCTURE_1le8| PDB=1le8 | SCENE= }}
-
|RELATEDENTRY=[[1yrn|1YRN]], [[1akh|1AKH]]
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1le8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1le8 OCA], [http://www.ebi.ac.uk/pdbsum/1le8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1le8 RCSB]</span>
+
-
}}
+
'''Crystal Structure of the MATa1/MATalpha2-3A Heterodimer Bound to DNA Complex'''
'''Crystal Structure of the MATa1/MATalpha2-3A Heterodimer Bound to DNA Complex'''
Line 29: Line 26:
[[Category: Vershon, A K.]]
[[Category: Vershon, A K.]]
[[Category: Wolberger, C.]]
[[Category: Wolberger, C.]]
-
[[Category: isothermal titration calorimetry]]
+
[[Category: Isothermal titration calorimetry]]
-
[[Category: matalpha2]]
+
[[Category: Matalpha2]]
-
[[Category: protein-dna complex]]
+
[[Category: Protein-dna complex]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:50:16 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:01:50 2008''
+

Revision as of 20:50, 2 May 2008

Image:1le8.jpg

Template:STRUCTURE 1le8

Crystal Structure of the MATa1/MATalpha2-3A Heterodimer Bound to DNA Complex


Overview

Triply mutated MATalpha2 protein, alpha2-3A, in which all three major groove-contacting residues are mutated to alanine, is defective in binding DNA alone or in complex with Mcm1 yet binds with MATa1 with near wild-type affinity and specificity. To gain insight into this unexpected behavior, we determined the crystal structure of the a1/alpha2-3A/DNA complex. The structure shows that the triple mutation causes a collapse of the alpha2-3A/DNA interface that results in a reorganized set of alpha2-3A/DNA contacts, thereby enabling the mutant protein to recognize the wild-type DNA sequence. Isothermal titration calorimetry measurements reveal that a much more favorable entropic component stabilizes the a1/alpha2-3A/DNA complex than the alpha2-3A/DNA complex. The combined structural and thermodynamic studies provide an explanation of how partner proteins influence the sequence specificity of a DNA binding protein.

About this Structure

1LE8 is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Structural and thermodynamic characterization of the DNA binding properties of a triple alanine mutant of MATalpha2., Ke A, Mathias JR, Vershon AK, Wolberger C, Structure. 2002 Jul;10(7):961-71. PMID:12121651 Page seeded by OCA on Fri May 2 23:50:16 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1le8"
Personal tools