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5e25

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Crystal structure of branched-chain aminotransferase from thermophilic archaea Geoglobus acetivorans complexed with alpha-ketoglutarate

Structural highlights

5e25 is a 3 chain structure with sequence from Geoglobus acetivorans. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.2Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

A0A0A7GJ30_GEOAI Acts on leucine, isoleucine and valine.[ARBA:ARBA00003109][RuleBase:RU364094]

Publication Abstract from PubMed

Two new thermophilic branched chain amino acid transaminases have been identified within the genomes of different hyper-thermophilic archaea, Geoglobus acetivorans, and Archaeoglobus fulgidus. These enzymes belong to the class IV of transaminases as defined by their structural fold. The enzymes have been cloned and over-expressed in Escherichia coli and the recombinant enzymes have been characterized both biochemically and structurally. Both enzymes showed high thermostability with optimal temperature for activity at 80 and 85 degrees C, respectively. They retain good activity after exposure to 50% of the organic solvents, ethanol, methanol, DMSO and acetonitrile. The enzymes show a low activity to (R)-methylbenzylamine but no activity to (S)-methylbenzylamine. Both enzymes have been crystallized and their structures solved in the internal aldimine form, to 1.9 A resolution for the Geoglobus enzyme and 2.0 A for the Archaeoglobus enzyme. Also the Geoglobus enzyme structure has been determined in complex with the amino acceptor alpha-ketoglutarate and the Archaeoglobus enzyme in complex with the inhibitor gabaculine. These two complexes have helped to determine the conformation of the enzymes during enzymatic turnover and have increased understanding of their substrate specificity. A comparison has been made with another (R) selective class IV transaminase from the fungus Nectria haematococca which was previously studied in complex with gabaculine. The subtle structural differences between these enzymes has provided insight regarding their different substrate specificities.

Thermostable Branched-Chain Amino Acid Transaminases From the Archaea Geoglobus acetivorans and Archaeoglobus fulgidus: Biochemical and Structural Characterization.,Isupov MN, Boyko KM, Sutter JM, James P, Sayer C, Schmidt M, Schonheit P, Nikolaeva AY, Stekhanova TN, Mardanov AV, Ravin NV, Bezsudnova EY, Popov VO, Littlechild JA Front Bioeng Biotechnol. 2019 Jan 24;7:7. doi: 10.3389/fbioe.2019.00007., eCollection 2019. PMID:30733943^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Isupov MN, Boyko KM, Sutter JM, James P, Sayer C, Schmidt M, Schonheit P, Nikolaeva AY, Stekhanova TN, Mardanov AV, Ravin NV, Bezsudnova EY, Popov VO, Littlechild JA. Thermostable Branched-Chain Amino Acid Transaminases From the Archaea Geoglobus acetivorans and Archaeoglobus fulgidus: Biochemical and Structural Characterization. Front Bioeng Biotechnol. 2019 Jan 24;7:7. doi: 10.3389/fbioe.2019.00007., eCollection 2019. PMID:30733943 doi:http://dx.doi.org/10.3389/fbioe.2019.00007

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5e25"

@@ Line 3: / Line 3: @@
 <StructureSection load='5e25' size='340' side='right'caption='[[5e25]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5e25]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Dsm_21716 Dsm 21716]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5E25 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5E25 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5e25]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Geoglobus_acetivorans Geoglobus acetivorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5E25 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5E25 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AKG:2-OXOGLUTARIC+ACID'>AKG</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[5cm0|5cm0]]</div></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AKG:2-OXOGLUTARIC+ACID'>AKG</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GACE_1900 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=565033 DSM 21716])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5e25 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5e25 OCA], [https://pdbe.org/5e25 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5e25 RCSB], [https://www.ebi.ac.uk/pdbsum/5e25 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5e25 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/A0A0A7GJ30_GEOAI A0A0A7GJ30_GEOAI] Acts on leucine, isoleucine and valine.[ARBA:ARBA00003109][RuleBase:RU364094]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 25: / Line 26: @@
 __TOC__
 </StructureSection>
-[[Category: Dsm 21716]]
+[[Category: Geoglobus acetivorans]]
 [[Category: Large Structures]]
-[[Category: Boyko, K M]]
+[[Category: Boyko KM]]
-[[Category: Mardanov, A V]]
+[[Category: Mardanov AV]]
-[[Category: Nikolaeva, A Y]]
+[[Category: Nikolaeva AY]]
-[[Category: Popov, V O]]
+[[Category: Popov VO]]
-[[Category: Rakitin, A L]]
+[[Category: Rakitin AL]]
-[[Category: Ravin, N V]]
+[[Category: Ravin NV]]
-[[Category: Stekhanova, T N]]
+[[Category: Stekhanova TN]]
-[[Category: Alpha-ketoglutarate]]
-[[Category: Aminotransferase]]
-[[Category: Bcat]]
-[[Category: Branched-chain]]
-[[Category: Complex]]
-[[Category: Geoglobus acetivoran]]
-[[Category: Plp]]
-[[Category: Transferase]]

5e25

From Proteopedia

Current revision

Crystal structure of branched-chain aminotransferase from thermophilic archaea Geoglobus acetivorans complexed with alpha-ketoglutarate

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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