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| | <StructureSection load='5e7q' size='340' side='right'caption='[[5e7q]], [[Resolution|resolution]] 2.23Å' scene=''> | | <StructureSection load='5e7q' size='340' side='right'caption='[[5e7q]], [[Resolution|resolution]] 2.23Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5e7q]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"streptomyces_platensis_subsp._rosaceus"_karwowski_et_al._1994 "streptomyces platensis subsp. rosaceus" karwowski et al. 1994]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5E7Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5E7Q FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5e7q]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_platensis_subsp._rosaceus Streptomyces platensis subsp. rosaceus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5E7Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5E7Q FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.23Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5e7q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5e7q OCA], [http://pdbe.org/5e7q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5e7q RCSB], [http://www.ebi.ac.uk/pdbsum/5e7q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5e7q ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5e7q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5e7q OCA], [https://pdbe.org/5e7q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5e7q RCSB], [https://www.ebi.ac.uk/pdbsum/5e7q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5e7q ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0A0A0V031_STRPT A0A0A0V031_STRPT] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Streptomyces platensis subsp. rosaceus karwowski et al. 1994]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Babnigg, G]] | + | [[Category: Streptomyces platensis subsp. rosaceus]] |
| - | [[Category: Chang, C Y]] | + | [[Category: Babnigg G]] |
| - | [[Category: Cuff, M E]] | + | [[Category: Chang CY]] |
| - | [[Category: Endres, M]] | + | [[Category: Cuff ME]] |
| - | [[Category: Hatzos-Skintges, C]] | + | [[Category: Endres M]] |
| - | [[Category: Joachimiak, A]] | + | [[Category: Hatzos-Skintges C]] |
| - | [[Category: Structural genomic]]
| + | [[Category: Joachimiak A]] |
| - | [[Category: Ma, M]] | + | [[Category: Ma M]] |
| - | [[Category: NatPro, Enzyme Discovery for Natural Product Biosynthesis]]
| + | [[Category: Osipiuk J]] |
| - | [[Category: Osipiuk, J]] | + | [[Category: Phillips Jr GN]] |
| - | [[Category: Phillips, G N]] | + | [[Category: Rudolf J]] |
| - | [[Category: Rudolf, J]] | + | [[Category: Shen B]] |
| - | [[Category: Shen, B]] | + | |
| - | [[Category: Acyl-coa synthetase]]
| + | |
| - | [[Category: Apc109894]]
| + | |
| - | [[Category: Enzyme discovery for natural product biosynthesis]]
| + | |
| - | [[Category: Mcsg]]
| + | |
| - | [[Category: Natpro]]
| + | |
| - | [[Category: Psi-biology]]
| + | |
| - | [[Category: Ptma2]]
| + | |
| - | [[Category: Transferase]]
| + | |
| Structural highlights
Function
A0A0A0V031_STRPT
Publication Abstract from PubMed
Acyl-coenzyme A (CoA) ligases catalyze the activation of carboxylic acids via a two-step reaction of adenylation followed by thioesterification. Here, we report the discovery of a non-adenylating acyl-CoA ligase PtmA2 and the functional separation of an acyl-CoA ligase reaction. Both PtmA1 and PtmA2, two acyl-CoA ligases from the biosynthetic pathway of platensimycin and platencin, are necessary for the two steps of CoA activation. Gene inactivation of ptmA1 and ptmA2 resulted in the accumulation of free acid and adenylate intermediates, respectively. Enzymatic and structural characterization of PtmA2 confirmed its ability to only catalyze thioesterification. Structural characterization of PtmA2 revealed it binds both free acid and adenylate substrates and undergoes the established mechanism of domain alternation. Finally, site-directed mutagenesis restored both the adenylation and complete CoA activation reactions. This study challenges the currently accepted paradigm of adenylating enzymes and inspires future investigations on functionally separated acyl-CoA ligases and their ramifications in biology.
Natural separation of the acyl-CoA ligase reaction results in a non-adenylating enzyme.,Wang N, Rudolf JD, Dong LB, Osipiuk J, Hatzos-Skintges C, Endres M, Chang CY, Babnigg G, Joachimiak A, Phillips GN Jr, Shen B Nat Chem Biol. 2018 Jul;14(7):730-737. doi: 10.1038/s41589-018-0061-0. Epub 2018 , Jun 4. PMID:29867143[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Wang N, Rudolf JD, Dong LB, Osipiuk J, Hatzos-Skintges C, Endres M, Chang CY, Babnigg G, Joachimiak A, Phillips GN Jr, Shen B. Natural separation of the acyl-CoA ligase reaction results in a non-adenylating enzyme. Nat Chem Biol. 2018 Jul;14(7):730-737. doi: 10.1038/s41589-018-0061-0. Epub 2018 , Jun 4. PMID:29867143 doi:http://dx.doi.org/10.1038/s41589-018-0061-0
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